COMPARISON OF POLYMORPHISM rs2070672 CYP2E1 GENE PROPORTION IN EARLY AND ADVANCED STAGE OF UNDIFFERENTIATED TYPE NASOPHARYNGEAL CARCINOMA IN BALINESE

Authors

  • I Ketut Suanda Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia https://orcid.org/0000-0002-2789-6607
  • I Gde Ardika Nuaba Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia https://orcid.org/0000-0002-7827-7195
  • Ni Putu Ayu Wiarni Susanthi Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia

DOI:

https://doi.org/10.32734/ijnpc.v2i01.2652

Keywords:

NPC, Balinese, polymorphism, rs2070672 CYP2E1 gene

Abstract

Introduction: Nasopharyngeal carcinoma is the most common malignancy in the ENT field. The cause of nasopharyngeal carcinoma is multifactorial. One of the risk factors for an increase in nasopharyngeal carcinoma is the rs2070672 polymorphism of the CYP2E1gene.

Purpose: To determine the rs2070672 CYP2E1gene polymorphism proportion in early and advanced stage undifferentiated  type NPC subjects in Balinese.

Method: This research is a cross sectional comparative study. The case population were all subjects with undifferentiated type NPC in the ENT outpatient clinic at Sanglah General Hospital Denpasar. This study uses 65 samples. Data collected in the form of subject characteristics and rs2070672 CYP2E1gene polymorphisms which examined by ARMS-PCR technique.

Results: The average age of the sample was 48.1 years, the most were male as many as 48 subjects (73.8%), and the highest was advanced stage as many as 56 subjects (86.2%). In the chi square test the proportion of polymorphisms in the advanced stage was 2.357 times higher than the early stages. The results of multivariate analysis using logistic regression proved that the rs2070672 CYP2E1gene polymorphism at advanced stage was 7.469 times higher than early stage.

Conclusion: There is a difference in the proportion of rs2070672 CYP2E1gene polymorphism in undifferentiated type NPC of Balinese, where advanced stage is higher than early stage.

Author Biographies

I Ketut Suanda, Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia

Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia Oncology Head and Neck division

I Gde Ardika Nuaba, Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia

Department of Otorhinolaryngology, Medical Faculty of Udayana University, Sanglah Hospital, Denpasar, Indonesia Oncology Head and Neck Division

References

[1] Duvvuri, U., Kubicek, G.J. 2014. Principles of Radiation Oncology. In : Johnson, J.T., Rosen, C.A., editors. Bailey’s Head and Neck Surgery Otolaryngology. 5th. Ed. Philadelphia:Lippincott Williams & Wilkins. p. 125-132.
[2] Murtiono W. 2013. Pengaruh Radioterapi Eksterna Terhadap Penurunan Kualitas Hidup Penderita Karsinoma Nasofaring WHO tipe III. Tesis. Bagian Ilmu Kesehatan Telinga Hidung Tenggorok Bedah Kepala Leher Fakultas Kedokteran Universitas Padjajaran. Bandung.
[3] Tsao, S.W., Yip, Y.L., Tsang, C.M., Pang, P.S., Lau, V.M.Y., Zhang, G., et al. 2014. Etiological Factors of Nasopharyngeal Carcinoma. Oral Oncol. 50:330-338.
[4] Danko, I.M., Chaschin, N.A. 2015. Association of CYP2E1 Gene Polymorphism with Predisposition to Cancer Development. Exp. Oncol. 27:248–256.
[5] Balitbang Kemenkes RI. 2013. Riset Kesehatan Dasar (RISKESDAS). Jakarta: Balitbang Kemenkes RI.
[6] Suhda, S., Paramita, D.K., Fachiroh, J. 2016. Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphisms of the CYP2E1 Gene. Asian Pac. J. Cancer Prev.. 17:3065-3069.
[7] Razek, A.A., King, A. 2012. MRI and CT of Nasopharyngeal Carcinoma. Am. J. Roent. 198:11-18.
[8] Cao, S., Simons, M., Qian, C. 2011. The Prevalence and Prevention of Nasopharyngeal Carcinoma in China. J. Chi. Anti-Can. 30:114-119.
[9] Turkoz, F.P., Celenkoglu, G., Dogu, G.G., Kalender, M.E., Coskun, U., Alkis, N. 2011. Risk Factors of Nasopharyngeal Carcinoma in Turkey – an Epidemiological Survey of the Anatolian Society of Medical Oncology. Asian Pac. J. Cancer Prev. 12: 3017-3021.
[10] Handayani, K., I.G.N.P. Putra, A.A.B.N. Nuartha. 2014. Kebutaan pada Karsinoma Nasofaring. CDK. 214:41-43.
[11] Adham, M., Kurniawan, A.N., Muhtadi, A.I., Roezin, A., Hermani, B., Gondhowiardjo, S., Tan, I.B., Middledorp, J.M. 2012. Nasopharyngeal Carcinoma in Indonesia: Epidemiology, Incidence, Signs, and Symptoms at Presentation. Chin. J. Cancer. 31:185-196.
[12] Sukardja, I.D.G. 2010. Onkologi Klinik. 2nd. Ed. Surabaya: Airlangga University Press. p.65.
[13] Nurdiansyah, F., Pardede, S.A., Farhat, Munir, D., Asnir, R.A., Hasibuan, M., Eyanoer, P.C. 2013. Korelasi Ekspresi Cyclooxygenase-2 (COX-2) dan TNF-α Sebagai Mediator Cancer-related Inflammation Pada Karsinoma Nasofaring. ORLI. 43:91-100.
[14] Hasibuan, N.R., Farhat, Haryuna, T.S.H., Yudhistira, A. 2014. Korelasi Positif Ekspresi Cyclooxygenase-2 dengan Microvessel Density pada Karsinoma Nasofaring. ORLI. p. 34-43.
[15] Cheng, D., Kong, H., Li, Y. 2014. Prognostic Value of Interleukin-8 and MMP-9 in Nasopharyngeal Carcinoma. Eur. Arch. Otorhinolaryngol. 271:2580.
[16] Faiza, S., Rahman, S., Asri, A. 2015. Karakteristik Klinis dan Patologis Karsinoma Nasofaring di Bagian THT-KL Dr. M. Djamil Padang. Jurnal Kesehatan Andalas. 5:90-95.
[17] Yin, Y.P. “Genetic Polymorphisms Associated With Nasopharyngeal Carcinoma Susceptibility in Malaysian Chinese” (disertasi). Kuala Lumpur: Universitas Malaya.
[18] Tang, K., Li, X., Xing, Q., Li, W., Feng, G., He, L., dkk. 2010. Genetic Polymorphism Analysis of Cytochrome P4502E1 (CYP2E1) in Chinese Han Populations from Four Different Geographic Areas of Mainland China. Genomics. 95:224-229.
[19] Wang, L., Ren, G., Li, J., Zhu, L., Niu, F., Yan, M., Li, J., Yuan, D., Jin, T. 2017. Genetic Polymorphism Analysis of Cytochrome P4502E1 (CYP2E1) in a Chinese Tibetan Population. Medicine. 96:47-51.
[20] Jia, W.H., Qin, H.D. 2012. Non-viral Environmental Risk Factors For Nasopharyngeal Carcinoma: A Systematic Review. Semin Cancer Biol. 22:117-126.

Published

2020-03-18

How to Cite

1.
Suanda IK, Nuaba IGA, Susanthi NPAW. COMPARISON OF POLYMORPHISM rs2070672 CYP2E1 GENE PROPORTION IN EARLY AND ADVANCED STAGE OF UNDIFFERENTIATED TYPE NASOPHARYNGEAL CARCINOMA IN BALINESE. IJNPC [Internet]. 2020Mar.18 [cited 2020May27];2(01):10-2. Available from: https://talenta.usu.ac.id/IJNPC/article/view/2652