Uji Toksisitas Akut Ekstrak Etanol Daun Sirsak (Annona muricata L.) Terhadap Ginjal Mencit
DOI:
https://doi.org/10.32734/idjpcr.v6i2.13724Abstract
Soursop leaf ethanol extract was reported to have immunomodulatory, antidiabetic, anti-inflammatory, lowering uric acid and cholesterol levels properties. Based on these reports, soursop leaf ethanol extract has the potential to be developed into a standardized herbal medicine. To ensure safety, this extract must undergo toxicity studies before marketing. This study examined soursop leaf ethanol extract acute toxicity. LD50 was estimated using the Reed and Muench method, and the effect of the extract on the kidneys was observed. This study used 50 mice—25 males and 25 females—divided into 5 groups. The control group received sodium carboxymethyl cellulose (CMC-Na) 0.5% w/v, and the treatment groups received ethanol extract of soursop leaves at doses of 500, 1000, 1500, and 2000 mg/kg bw orally on the first day, then body weight and number of deaths were calculated and the toxic symptoms were observed over 14 days. After 14 days, the surviving mice were sacrificed to examine the relative organ weights, macropathology and histopathology of the mice kidneys. Male and female mice in the 1500 and 2000 mg/kg bw dose groups showed toxic symptoms. There was no significant difference in relative organ weight at 14 days between groups. The results of organ macropathology showed no colour differences in all groups. Based on histopathological examination, mild damage to the kidney organs can be seen at doses of 1500 and 2000 mg/kg bw and the LD50 result was 1819 mg/kg bw. In conclusion, soursop leaf ethanol extract has a mild toxic effect on mice.
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References
Mardiana L, Ratnasari J. Ramuan dan khasiat sirsak. Penebar Swadaya Grup; 2011.
Zuhud E.A.M. Bukti kedahsyatan: sirsak menumpas kanker. AgroMedia; 2011.
Adedapo, A. A., Oni, O. A., Falayi, O. O., Ogunmiluyi, I. O., Ogunpolu, B. S., Omobowale, T. O., ... & Yakubu, M. A. (2022). Annona muricata mitigates glycerol-induced nephrotoxicities in male albino rats through signaling pathways of angiotensin conversion enzyme, kidney injury molecule-1, and antioxidant properties. Scientific African, 16, e01225.
Zeweil, M. M., Khafaga, A. F., Mahmoud, S. F., Wasef, L., Saleh, H., Elrehim, A. M. A., ... & Ghoneim, H. A. (2024). Annona Muricata L. extract restores renal function, oxidative stress, immunohistochemical structure, and gene expression of TNF-α, IL-β1, and CYP2E1 in the kidney of DMBA-intoxicated rats. Frontiers in Pharmacology, 15, 1348145.
Dewi LK, Widyarti S. Pengaruh pemberian ekstrak Etanol daun Sirsak (Annona muricata Linn.) terhadap peningkatan jumlah sel T CD4+ dan CD8+ pada Timus Mencit (Mus musculus). Biotropika: Journal of Tropical Biology. 2013 May 1;1(1):24-6.
Pandaleke SS, de Queljoe E, Abdullah SS. Uji Efektivitas Ekstrak Etanol Daun Sirsak (Annona muricata L.) Untuk Menurunkan Kadar Gula Darah Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Pharmacon. 2022 Feb 24;11(1):1321-7.
Rahmawati R, Rahman S, Mustari M. Uji Efek Antiinflamasi Ekstrak Etanol Daun Sirsak (Annona muricata Linn.) Terhadap Mencit (Mus musculus) Jantan Yang Diinduksi Dengan Karagen. As-Syifaa Jurnal Farmasi. 2012 Jul 1;4(1):7-15.
Tayeb R, Ubyaan M, Usmar U. Uji Efek Pemberian Ekstrak Etanol Daun Sirsak (Anonna muricata L.) terhadap Kadar Asam Urat pada Tikus Putih (Rattus norvegicus). In Proceeding of Mulawarman Pharmaceuticals Conferences 2016 Apr 26 (Vol. 3, pp. 367-373).
Florencia E, Ginting B. Uji Efek Ekstrak Etanol Daun Sirsak (Annona Muricata L,) Terhadap Penurunan Kadar Kolesterol Pada Kelinci Dengan Atorvastatin Sebagai Pembanding. Politeknik Kesehatan Medan. 2022.
Sari, Sudarmi, Patilaya P. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirsak Muda dan Tua (Annona muricata L.) Terhadap Staphylococcus aureus (Skripsi). Medan: Fakultas Farmasi Universitas Sumatera Utara. 2018.
Casarett LJ. Casarett and Doull's toxicology: The basic science of poisons. New York: McGraw-Hill; 2008.
Lu FC, Toksikologi Dasar: Asas, Organ Sasaran, dan Penilaian Risiko. Diterjemahkan oleh Nugroho E, Bustami ZS, dan Darmansjah I. Edisi Kedua. Jakarta: UI Press. 1994.
Priyanto SH. Toksikologi mekanisme, terapi antidotum dan penilaian risiko. Yogyakarta: Leskonfi. 2009.
Robbins dan Cotran. Pathology Basic of Disease. Edisi Ketujuh. New York: Elsevier, Inc. 2009. Halaman 977-981.
World Health Organization (WHO). Quality control methods for medicinal plant materials. World Health Organization; 1998.
DepKes RI. Materia Medika Indonesia Jilid VI, Cetakan Keenam. Jakarta: Direktorat Jenderal Pengawasan Obat Dan Makanan. 1995:92-4.
Reed LJ, Muench H. A simple method of estimating fifty percent endpoints. American journal of epidemiology. 1938 May 1;27(3):493-7.
Angelina M, Hartati S, Dewijanti ID, Banjarnahor SD, Meilawati L. Penentuan LD50 Daun Cinco (Cyclea barbata Miers.) pada Mencit. Makara Journal of Science. 2010 Oct 14.
Organization for economic cooperation and development (OECD). Guidelines for the Testing of Chemicals, OECD 407. Acute Oral Toxicity-Acute Toxic Class Method.
Lu FC, Toksikologi dasar: asas, organ sasaran, dan penilaian resiko. Edisi II. Jakarta. UIP. 1995. Hal. 85-86, 206-220.
Ditjen POM, Depkes RI. Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan Republik Indonesia. 2000:10-5.
Depkes RI. Pedoman pelaksanaan uji klinik obat tradisional. Jakarta: DepKes RI, DitJen POM, Direktorat Pengawasan Obat Tradisional. 2000.
Rahman, Friska Ani, Tetiana Haniastuti, and Trianna Wahyu Utami. "Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668." Majalah Kedokteran Gigi Indonesia 3.1 (2017): 1-7.
Lazarovici P, Laberbourn-Galski H. Chimeric tocin. Mechanisms of actions and theraupeutic applications. New York: CRC Press. 2002.
Syamsuni dan Darmono. Buku ajar farmakologi experimental. Jakarta: UI Press. 2011. Hal. 46.
Ditjen POM, Depkes RI. Pedoman Uji Toksisitas Nonklinik Secara In Vivo. 2014.
Bhardwaj S, Gupta D. Study of acute, sub-acute and chronic toxicity test. International Journal of Advanced Research in Pharmaceutical & Bio Sciences. 2012 Apr 1;1(3):103-30.
Ar Razi F, Uji Toksisitas Akut Ekstrak Etanol Bunga Pepaya Jantan (Carica papaya L.) pada Mencit Jantan (Skripsi). Medan: Fakultas Farmasi Universitas Sumatera Utara. 2014.
Anggraini DR, Gambaran makroskopik hati dan ginjal mencit akibat pemberian plum asetat (Tesis). Medan: Sekolah Pascasarjana Universitas Sumatera Utara. 2008.
Setiati N. Pemberian Ekstrak Benalu Mangga terhadap Perubahan Histologis Hepar Tikus yang Diinduksi Kodein. Biosaintifika: Journal of Biology & Biology Education. 2014;6(2):80-6.
Juhryyah, S. Gambaran Histopatologi Organ Hati dan Ginjal Tikus Intoksikasi Akut Insektisida (Metofluthrin, D-Phenothrin, D-Allethrin) dengan Dosis Bertingkat (Skripsi). Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor; 2008.
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