Test of Burn Wounds Healing Effects of Collagen From Snakehead Fish (Channa striata) Bone in The Preparation of Cream on Male White Rats (Rattus norvegicus)
DOI:
https://doi.org/10.32734/idjpcr.v3i1.3977Abstract
Abstract. The use of snakehead fish (Channa striata) collagen in cream is an alternative to
reduce fish wastes. It has been proved that snakehead fish (Channa striata) contain collagen.
This research aims to study the burn wound healing effectivity of bone’s collagen from
snakehead fish (Channa striata) that is formulated into a cream. This study used the
experimental method with the bone of snakehead fish (Channa striata) as a sample. This study
consisted of 4 (four) such as isolation of collagen from snakehead fish's (Channa striata) bone,
characterization of collagen by analyzing moisture, ash, protein, fat and functional group
analysis with FTIR, formulation, and evaluations of cream such as organoleptic test,
homogeneity test, pH measurement, stability test, and irritation test, and burn wound healing
test in male white rats (Rattus norvegicus) and the result is analyzed using SPSS 22.0 Free trial
and One Way ANOVA and Post-Hoc Tukey HSD. The results showed that fish collagen could
be isolated from snakehead fish (Channa striata) and the yield obtained is 33.3%. The results of
collagen characterization and evaluation test met the collagen standard requirements. Results of
the burn wound healing test on male white rats (Rattus norvegicus) showed that K1 burn wound
recovered on day 21, K2 on day 12, K3 on day 15, K4 on day 18, and K5 on day 18.
Measurement of burn wound diameter on day 21 showed K1 = 1.20 cm, K2 = 0.15 cm, K3 =
0.10 cm, K4 = 0.45 cm, K5 = 0.40 cm. The results of the statistical analysis of burn wound
diameter showed a significant difference p = 0,000 (p <0.05) between each group. K3 showed
the reduction in the burn wound diameter is faster and the smallest, it can be concluded that the
optimal dose of snakehead fish bone’s collagen cream is a concentration of 3%.
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