Pengaruh Konsentrasi Enzim, Ragi, dan Lama Fermentasi terhadap Pembuatan Bioetanol Berbahan Baku Chlorella pyrenoidosa

Asyeni Miftahul  Jannah; Indah Wahyuni; Renanda Amalia; Cynthia Savrinda; Indry Permata  Hani

doi:10.32734/jtk.v13i2.15601

Authors

Asyeni Miftahul Jannah Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sriwijaya
Indah Wahyuni Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sriwijaya
Renanda Amalia Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sriwijaya
Cynthia Savrinda Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sriwijaya
Indry Permata Hani Jurusan Teknik Kimia, Fakultas Teknik, Universitas Sriwijaya

DOI:

https://doi.org/10.32734/jtk.v13i2.15601

Keywords:

bioethanol, chlorella pyrenoidosa, hydrolysys, fermentation, α-amilase enzyme

Abstract

The demand for energy is inversely proportional to the current amount of energy in Indonesia. In order to face this problem, it is necessary to develop renewable energy sources. This study was conducted to analyze enzymes effect in the Chlorella pyrenoidosa hydrolysis, analyze yeast effect and the fermentation time of Chlorella pyrenoidosa on the bioethanol produced. The variables used are variation of α-amylase enzyme concentration of 25%, 35%, 45% (v/w), and variation of yeast concentration of 5%, 10%, 15% (w/v) and fermentation time of 7, 9, 11 days. From the analysis conducted, the highest glucose content was 13.119%, which was in the variation of 45% (v/w) α-amylase enzyme concentration. The amount of enzyme concentration used was in accordance with glucose production. The highest bioethanol was obtained with a sample involving 10% (v/b) yeast fermented for 9 days, which amounted to 27%.

Downloads

Download data is not yet available.

References

P. R. Indonesia, “Peraturan Pemerintah No. 79 Thn 2014 tentang energi alternatif,” Indonesia, pp. 1–36, 2014.

S. O. Gultom, “Mikroalga: sumber energi terbarukan masa depan,” J. Kelaut. Indones. J. Mar. Sci. Technol., vol. 11, no. 1, p. 95, 2018, doi: 10.21107/jk.v11i1.3802.

A. Y. M. Simanjuntak and R. Subagyo, “Analisis hasil fermentasi pembuatan bioetanol dengan variasi waktu menggunakan bahan (singkong, beras ketan hitam dan beras ketan putih),” Sci. J. Mech. Eng. Kinemat., vol. 4, no. 2, pp. 79–90, 2019, doi: 10.20527/sjmekinematika.v4i2.119.

J. Velazquez-Lucio et al., “Microalgal biomass pretreatment for bioethanol production: A review,” Biofuel Res. J., vol. 5, no. 1, pp. 780–791, 2018, doi: 10.18331/BRJ2018.5.1.5.

D. P. Yuniarti, S. Hatina, and W. Efrinalia, “Pengaruh Jumlah ragi dan waktu fermentasi pada pembuatan bioetanol dengan bahan baku ampas tebu,” J. Redoks, vol. 3, no. 2, pp. 1–12, 2018, doi: 10.31851/redoks.v3i2.2391.

K. Rueangsan, S. Trisupakitti, W. Senajuk, and J. Morris, “Fast Pyrolysis of dipterocarpus alatus roxb and rubber wood in a free-fall reactor,” Energy Sources, Part A Recover. Util. Environ. Eff., vol. 44, no. 1, pp. 2489–2496, 2022, doi: 10.1080/15567036.2019.1649760.

M. Melikoglu and B. Turkmen, “Food waste to energy: Forecasting Turkey’s bioethanol generation potential from wasted crops and cereals till 2030,” Sustain. Energy Technol. Assessments, vol. 36, no. October, p. 100553, 2019, doi: 10.1016/j.seta.2019.100553.

K. Tolulope Eunice, “Enzymatic and dilute acid hydrolyses of maize stalk substrate in bio-ethanol production,” J. Energy, Environ. Chem. Eng., vol. 6, no. 1, pp. 24–30, 2021, doi: 10.11648/j.jeece.20210601.14.

C. E. de Farias Silva and A. Bertucco, “Bioethanol from microalgal biomass: A promising approach in biorefinery,” Brazilian Arch. Biol. Technol., vol. 62, pp. 1–14, 2019, doi: 10.1590/1678-4324-2019160816.

J. Damaurai, T. Preechakun, M. Raita, V. Champreda, and N. Laosiripojana, “Investigation of alkaline hydrogen peroxide in aqueous organic solvent to enhance enzymatic hydrolysis of rice straw,” Bioenergi Res., 2020, doi: https://doi.org/10.1007/s12155-020-10152-5.

M. Yerizam et al., “Bioethanol production from coconut husk using DES-NADES pretreatment and enzymatic hydrolysis method,” Comptes Rendus Chim., vol. 15, pp. 1060–1064, 2023, [Online]. Available: http://dx.doi.org/10.1016/j.crci.2012.09.005.

S. Polprasert, O. Choopakar, and P. Elefsiniotis, “Bioethanol production from pretreated palm empty fruit bunch (PEFB) using sequential enzymatic hydrolysis and yeast fermentation,” Biomass and Bioenergy, vol. 149, no. April, pp. 1–9, 2021, doi: 10.1016/j.biombioe.2021.106088.

P. Selvakumar et al., “Optimization of binary acids pretreatment of corncob biomass for enhanced recovery of cellulose to produce bioethanol,” Fuel, vol. 321, no. April, p. 124060, 2022, doi: 10.1016/j.fuel.2022.124060.

M. Takano and K. Hoshino, “Bioethanol production from rice straw by simultaneous saccharification and fermentation with statistical optimized cellulase cocktail and fermenting fungus,” Bioresour. Bioprocess., vol. 5, no. 1, 2018, doi: 10.1186/s40643-018-0203-y.

E. A. Beltagy, A. Abouelwafa, and K. M. Barakat, “Bioethanol production from immobilized amylase produced by marine Aspergillus flavus AUMC10636,” Egypt. J. Aquat. Res., no. xxxx, 2022, doi: 10.1016/j.ejar.2022.02.003.

M. J. Nuhma, H. Alias, A. A. Jazie, and M. Tahir, “Role of microalgae as a source for biofuel production in the future: A short review,” Bull. Chem. React. Eng. Catal., vol. 16, no. 2, pp. 396–412, 2021, doi: 10.9767/bcrec.16.2.10503.396-412.

L. Arlianti, “Bioetanol sebagai sumber green energy alternatif yang potensial di Indonesia,” Unistek, vol. 5, no. 1, pp. 16–22, 2018, doi: 10.33592/unistek.v5i1.280.

B. Cochran, D. Lunday, and F. Miskevich, “Kinetic analysis of amylase using quantitative Benedict’s and iodine starch reagents,” J. Chem. Educ., vol. 85, no. 3, pp. 401–403, 2008, doi: 10.1021/ed085p401.

N. A. Arifiyanti, D. N. Aqliyah, and M. Billah, “Bioetanol dari biji nangka dengan proses likuifikasi dan fermentasi menggunakan Saccharomyces cerevisiae,” ChemPro, vol. 1, no. 1, pp. 51–55, 2020, doi: 10.33005/chempro.v1i01.47.

D. Istia’nah, U. Utami, and A. Barizi, “Karakterisasi Enzim amilase dari bakteri Bacillus megaterium pada variasi suhu, pH dan konsentrasi Substrat,” J. Ris. Biol. dan Apl., vol. 2, no. 1, p. 11, 2020, doi: 10.26740/jrba.v2n1.p11-17.

M. Yerizam, A. M. Jannah, and N. Aprianti, “Bioethanol production from chlorella pyrenoidosa by using enzymatic hydrolysis and fermentation method,” J. Ecol. Eng., vol. 24, no. 1, pp. 34–40, 2023, doi: 10.12911/22998993/156000.

I. Febriana, Zurohiana, A. Zikri, and S. Hatina, “Effect of bread yeast (Saccharomyces Cereviseae) concentration and fermentation time in the manufacture of bioethanol using banana peel,” Distilasi, vol. 3, no. 1, pp. 1–7, 2018.