Ekstraksi Tanin dari Buah Balakka (Phyllanthus emblica L.) dengan Bantuan Microwave: Pengaruh Daya Microwave, Perbandingan Massa Kering Terhadap Jumlah Pelarut Etil Asetat
DOI:
https://doi.org/10.32734/jtk.v10i1.5318Keywords:
ethyl acetate, Indian gooseberry, tanninAbstract
Tannins are phenolic compounds with considerable abundance in nature. In this study, the extraction of Indian gooseberry fruit was performed using Microwave Assisted Extraction (MAE) method with ethyl acetate as solvent. Two grams of Indian gooseberry fruit were extracted for 1 minute with variable microwave power operation (100, 180, 300, 450 and 600) W. The experiment is continued at the power that gave the highest yield of tannin with variable ratio sample to solvent (1/10, 1/20, 1/30, 1/40 and 1/50) g/mL. Total tannins in the extract were analyzed using UV-Vis Spectroscopy method to find the yield of tannin. Functional groups that were contained in the extract were analyzed using Fourier-Transform Infrared Spectroscopy (FTIR) method. The results of this study indicate that the extract contains tannins in qualitative tests with gelatin and FeCl3 as well as in FTIR analysis. The highest yield of tannin was 36.86 mg/g which was obtained under conditions of power 100 W and ratio sample with ethyl acetate 1/50 g/mL.
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References
M. R. Hasan, M. N. Islam, and M. R. Islam, “Phytochemistry, pharmacological activities and traditional uses of Emblica officinalis: A review,” Int. Curr. Pharm. J., vol. 5, no. 2, pp. 14–21, 2016.
N. K. Charmkar and R. Singh, “Emblica officinalis Gaertn. (Amla): A Wonder Gift of Nature to Humans,” Int. J. Curr. Microbiol. Appl. Sci., vol. 6, no. 7, pp. 4267–4280, 2017.
Y. Li et al., “Characterization of phenolic compounds from Phyllanthus emblica fruits using HPLC-ESI-TOF-MS as affected by an optimized microwave-assisted extraction,” Int. J. Food Prop., vol. 22, no. 1, pp. 330–342, 2019.
M. Llompart, C. Garcia-Jares, M. Celeiro, and T. Dagnac, Extraction | Microwave-Assisted Extraction, 3rd ed., Elsevier Inc., 2019.
U. Khoiriyah, N. Pasaribu, and S. Hannum, “Distribusi Phyllanthus emblica L. di Sumatera Utara Bagian Selatan,” Biosfera, vol. 32, no. 2, p. 98, 2015.
E. Lamy et al., “Determinants of tannin-rich food and beverage consumption: Oral perception vs. psychosocial aspects,” Tann. Biochem. Food Sources Nutr. Prop., pp. 29–58, 2016.
Q. Huang, X. Liu, G. Zhao, T. Hu, and Y. Wang, “Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production,” Anim. Nutr., vol. 4, no. 2, pp. 137–150, 2018.
A. Pandey, S. Tripathi, and C. A. Pandey, “Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug,” J. Pharmacogn. Phytochem. JPP, vol. 115, no. 25, pp. 115–119, 2014.
M. S. H. Putri, “Pengaruh H2SO4 dan KOH pada analisis Cr (III) menggunakan asam tanat secara Spektrofotometri Ultraungu – Tampak”, Skripsi, Universitas Lampung, Lampung, 2016.
R. Ahmad, G. Parveen, N. A. Gauri, and N. Wal, “Phytochemical screening, sugar content, total protein and antimicrobial activity of three important medicinal plants,” Med. Plant, vol. 5, no. 6, pp. 125–139, 2017.
M. A. Bharudin, S. Zakaria, and C. H. Chia, “Condensed tannins from Acacia mangium bark: Characterization by spot tests and FTIR,” AIP Conf. Proc., vol. 1571, pp. 153–157, 2013.
S. D. Marliana, V. Suryanti, and Suyono, “Skrining fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam ( Sechium edule Jacq . Swartz .) dalam ekstrak etanol,” Biofarmasi, vol. 3, no. 1, pp. 26–31, 2005.
E. Lembong, G. L. Utama, and R. A. Saputra, “Phytochemical test, vitamin c content and antioxidant activities beet root (Beta vulgaris Linn.) extracts as food coloring agent from some areas in java island,” IOP Conf. Ser. Earth Environ. Sci., vol. 306, no. 1, 2019.
T. Wahyono, D. A. Astuti, I. Komang Gede Wiryawan, I. Sugoro, and A. Jayanegara, “Fourier Transform Mid-Infrared (FTIR) Spectroscopy to identify tannin compounds in the panicle of Sorghum mutant lines,” IOP Conf. Ser. Mater. Sci. Eng., vol. 546, no. 4, 2019.
B. Buanasari, W. T. Eden, and A. I. Sholichah, “Extraction of phenolic compounds from petai leaves (Parkia Speciosa Hassk.) Using microwave and ultrasound assisted methods,” J. Bahan Alam Terbarukan, vol. 6, no. 1, pp. 25–31, 2017.
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