Effect Of Bangun-Bangun Leaves Ethanol Extract On Lipid Profile Ovariectomized Female Rats
DOI:
https://doi.org/10.32734/idjpcr.v4i1.5360Keywords:
Plectranthus amboinicus (Lour.) Spreng, Phytoestrogens, Ovariectomy, Lipid Profile, 17β-estradiolAbstract
Menopause was a condition of the body when ovaries stopped producing ovum; ovum is the main producer of estrogen hormone. Menopause can increasing lipid profile in blood; it causes any problems in the cardiovascular system. People of North Sumatra have long used Bangun-Bangun leaves as a breastfeeding agent; it contains flavonoid bioactive compounds, which are be expected to replace lost estrogen hormone. This study determined the estrogenic activity of Bangun-Bangun leaves ethanol extract (BBLEE) on lipid levels of ovariectomized female rats as a menopausal model. BBLEE has obtained by maceration techniques with 96% ethanol and extract used on 24 ovariectomized rats which were divided into 6 groups. Group 1 (normal), group 2 (positive) was treated by estradiol at dose of 0.18 mg/kg BW, group 3 (negative) was treated by 0.5% Na-CMC and group 4, 5, 6 were treated by BBLEE at dose of 30, 60, 90 mg/kg BW given orally for 14 days. The data were analyzed by ANOVA and Post Hoc Tukey HSD test. The result showed that effective dose of BBLEE was 90 mg/kg BW which improved lipid profile (TC = 55.75±0.47; TG = 63.00±0.40; HDL = 37.00±0.81; LDL = 30.50±0.86) in blood of ovariectomized female rats as a menopausal model. BBLEE at the dose of 90 mg/kg BW can ameliorate the lipid profile in the blood of ovariectomized female rats as a menopausal model.
Downloads
References
P.Anagnostis, C. John , Stevenson, D. Crook, G. Desmond, Johnston, F. Ian, Godsland, "Effects Of Menopause, Gender And Age On Lipids And High-Density Lipoprotein Cholesterol Subfractions" Clinical Investigations and Research Unit, Royal Sussex County Hospital, Sussex, School of Health Sciences, University of Brighton, UK., 2015.
Antonelli. A, Giannini. A, Caretto. M, Simoncini. T, Andrea. R. G, "Impact of Menopause on Brain Functions" Division of Obstetrics and Gynecology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy, 2019.
Armstrong. K, Schwartz. J.S, Randall. T, Rubin. S.C, Weber. B, "Hormone Replacement Therapy and Life Expectancy After Prophylactic Oophorectomy in Women With BRCA1/2 Mutations: A Decision Analysis" J Clin Oncol. 22 (6):1045–1054, 2004.
Arumugam. G, Swamy. M, Sinniah. U, "Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance". Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Malaysia, 2016.
Avis. N.E, "Duration of Menopausal Vasomotor Symptoms Over the Menopause Transition" American Medical Association. 175(4):531-539, 2015
Ditjen. P.O.M, "Materia Medika Indonesia. Jilid VI" Jakarta : Departemen Kesehatan RI, 1995.
Dixon. R, "Phytoestrogens". Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401; Centre for Novel Agricultural Products, University of York, Heslington Road, York, United Kingdom, 2004.
Farquhar. C. M, Sadler. L, Harvey. S. A, Stewart. A. W, "The association of hysterectomy and menopause: a prospective cohort study" BJOG: An International Journal of Obstetrics and Gynaecology. 112(7), 956–962, 2005.
Finch. C.E, " The Menopause and Aging, A Comparative Perspective" J Steroid Biochem Mol Biol. 142:132–141, 2014.
Simoncini. T, Hafezi-Moghadam. A, Brazil. D.P, Ley. K, Chin. W.W, Liao. J.K, "Interaction of Oestrogen Receptor with The Regulatory Subunit of Phosphatidylinositol-3-OH Kinase" Nature. 407:538 –541, 2000.
Nogowski. L, Mac kowiac. P, Kandulska. K, Szkudelski. T, Nowak. K.W, "Genistein-Induced Changes in Lipid Metabolism of Ovariectomized Rats" Ann Nutr Metab. 42:360–366, 1998.
Prangdimurti. E, Palupi. N.S, dan Zakaria. F.R, "Metoda Evaluasi Nilai Biologis Karbohidrat dan Lemak". Modul e-Learning ENBP. Bandung: Departemen Ilmu Teknologi Pangan, 2007.
Schlecht. C, Holger. K, Hubertus. J, Wolfgang. W, "Effects of Estradiol, Benzophenone-2 and Benzophenone-3 on The Expression Pattern of The Estrogen Receptors (ER) Alpha and Beta, The Estrogen Receptor-Related Receptor 1 (ERR1) and The Aryl Hydrocarbon Receptor (AHR) IN Adult Ovariectomized Rats" Volume 205, Issues 1–2, 1 December 2004.
Shuster, L.T, Rhodes. D.J, Gostout. B.S, Grossardt. B.R, Rocca. W.A, "Prematuremenopause or Early Menopause: Long-Term Health Consequences" Maturitas. 65,161–166, 2010.
Utian. W. H, "Menopause-Related Definitions" The North American Menopause Society. 1266 : 133-138, 2004.
Yoshida. T, Takahashi. K, Yamatami. H, Takata. K, Kurachi. H, "Impact of Surgical Menopause On Lipid and Bone Metabolism" Climacteric. 14:445-452, 2011.
Sikoski. P, Register. T. C, Lees. C. J, Lundeen. S, Hutchison. J, Brown. K. H, Cline. J. M, "Effects Of Two Novel Selective Estrogen Receptor Modulators, Raloxifene, Tamoxifen, And Ethinyl Estradiol On The Uterus, Vagina And Breast In Ovariectomized Cynomolgus Monkeys (Macaca fascicularis)" American Journal of Obstetrics and Gynecology, 196(1), 75.e1–75.e7. doi:10.1016/j.ajog.2006.09.038, 2007.
Sophocleous. A, Landao-Bassonga. E, van‘t Hof. R. J, Idris. A. I, Ralston. S. H, "The Type 2 Cannabinoid Receptor Regulates Bone Mass and Ovariectomy-Induced Bone Loss by Affecting Osteoblast Differentiation and Bone Formation" Endocrinology, 152(6), 2141–2149. doi:10.1210/en.2010-0930, 2011.
Idris. Aymen, "Ovariectomy / Orchidectomy in Rodents: Methods in Molecular Biology (Clifton, N.J)" Pubmed. The University of Sheffield. DOI: 10.1007/978-1-61779-415-5_34, 2012.
Cho. Sung-Hee, Lee. Hyang-Rim, Kim. Tae-Bee, Choi. Sang-Won, Lee. Won-Jung, Cho, Youngsun, "Effects of Defatted Safflower Seed Extract and Phenolic Compounds in Diet on Plasma and Liver Lipid in ovariectomized Rats Fed High-Cholesterol Diets" J Nutr Sci Vitaminol. 50 32-3 7, 2010.
Iwansyah. A, Damanik. R, Kustiyah. L, Hanafi. M, "Relationship Between Antioxidant Properties And Nutritional Composition Of Some Galactopoietics Herbs Used In Indonesia: A Comparative Study" Research Center for Chemistry, Indonesian Institute of Sciences, South Tangerang, 2016.
Iwansyah. A, Damanik. R, Kustiyah. L, Hanafi. M, "The Ethyl Acetate Fraction of Torbangun (Coleus amboinicus L.) Leaves Increasing Milk Production with Up-Regulated Genes Expression of Prolactin Receptor" Research Center for Chemistry. Vol. 9, No. 2, 147 – 154, 2019.
Published
How to Cite
Issue
Section
Copyright (c) 2021 Indonesian Journal of Pharmaceutical and Clinical Research
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR) and Faculty of Pharmacy as well as TALENTA Publisher Universitas Sumatera Utara as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, will be allowed only with a written permission from Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR).
The Copyright Transfer Form can be downloaded here.
The copyright form should be signed originally and sent to the Editorial Office in the form of original mail or scanned document.