Effect of Plectranthus amboinicus, Lour. Spreng. n-Hexane Extract on T47D Cells Line: Proliferation, Apoptosis and It’s Combination with Doxorubicine

Authors

  • Poppy Anjelisa Zaitun Hasibuan University of Sumatera Utara
  • Rosidah Departement Pharmacology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Pandapotan Nasution Departement Biology Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Syafruddin Ilyas Departement of Biology, Faculty of Mathematic and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.32734/idjpcr.v3i1.3323

Keywords:

acrydine orange, chemoprevention, combination index, doubling time, ethidium bromide, IC50 value ≤ 100 µg/mL, acrydine orange, chemoprevention, combination index, doubling time, ethidium bromide, IC50 value ≤ 100 µg/mL.

Abstract

The objectives of the study are to investigated the growth-inhibiting and apoptosis mediating effect of Plectranthus amboinicus (Lour.) Spreng n-hexane extract (PANE) on T47D cell lines. The assays were performed in the study were cytotoxicity assay and apoptosis induction of T47D cells. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis were observed by ethidium bromide-acrydine orange method. The growth of T47D was inhibited by treatment with PANE on concentrations 20; 40; dan 80 µg/mL. The cell death (apoptosis) induced by PANE was characterized by orange fluorescent on the IC50 concentration. These results concluded that the n-hexane extract of Plectranthus amboinicus, (Lour.) Spreng. inhibited the growth of T47D breast cancer cells in dose and time dependent manners. PANE also could induced apoptosis on T47D breast cancer cells, but the combination of PANE-doxorubicine did not show the synergistic effect. The results suggesting that PANE may be a potent agent for the chemoprevention of breast cancer.

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Published

2020-07-28

How to Cite

Zaitun Hasibuan, P. A., Rosidah, Nasution , P. ., & Ilyas , S. (2020). Effect of Plectranthus amboinicus, Lour. Spreng. n-Hexane Extract on T47D Cells Line: Proliferation, Apoptosis and It’s Combination with Doxorubicine. Indonesian Journal of Pharmaceutical and Clinical Research, 3(1), 8-17. https://doi.org/10.32734/idjpcr.v3i1.3323

Issue

Vol. 3 No. 1 (2020): Indonesian Journal of Pharmaceutical and Clinical Research

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