Evaluation of Acute Toxicity of Ethanol Extract of Pirdot Leaf (Saurauia vulcani Korth.) in Rats

Authors

  • Nerly Juli Pranita Simanjuntak Postgraduate Programs Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Rosidah Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia
  • Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, 20155, Indonesia

DOI:

https://doi.org/10.32734/idjpcr.v3i2.4303

Keywords:

Saurauia vulcani Korth , Acute toxicity, LD50,Hematological parameters,

Abstract

Traditionally pirdot leaves are used to treat various diseases. The purpose of this study was to determine determine the potential for acute toxicity of ethanolic extract of pirdot leaf (Saurauia vulcani Korth.) with value LD50 and hematological Parameters in rats. The acute toxicity of ethanolic extract of pirdot leaf was evaluated by OECD guidelines. The number of animals used in this research were 15 female rats. The control group was given Na CMC 0.5%, the treatment groups were given ethanolic extract of pirdot leaves with doses 2000 and 5000 mg/kg bw. The results showed that ethanolic extract of pirdot leaves with doses of 2000 and 5000 mg/kg bw did not show any toxicity signs. There was no mortality was observe. The ethanolic extract of pirdot leaves did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05). It was estimated that LD50 of ethanolic extract of pirdot leaves was higher than 5000 mg/kg bw and the extract were practically non-toxic. The ethanolic extract of pirdot leaves did not cause any toxic effect on hematological parameters.

Downloads

Download data is not yet available.

References

Ditjen, PEN., Kementrian Perdagangan Republik Indonesia. (2014). Warta Ekspor : Menyibak Potensi pasar Obat Tradisional. Ditjen PEN/MJL/005/9/2019 September.

Situmorang, R.O.P., Harianja, A.H., and Silalahi, J. (2015). Karo’s Local Wisdom: The Use Of Woody Plants For Traditional Diabetic Medicines. Indonesian Journal Of Forestry Research. 2(2): 121-131

Sitorus, P. (2015). Characterization Simplisia and Ethanolic Extract of Pirdot (Saurauia Vulcani, Korth) Leaves and Study of Antidiabetic Effect in Alloxan Induced Diabetic Mice. International Journal of ChemTech Research. 8(6):789-794.

Murakami, A. (2014). Dose-Dependent Functionality and Toxicity of Green Tea Polyphenols in Experimental Rodents. Elsevier. 557: 3-10

Man,S., Jing,L., dan Hongyan, C.,(2015). CurcuminAlleviated the Toxic Reaction of Rhizome Paridis Saponins in a 45-dy Subchronic Toxicological Assess,ent of rats. Willey Online Library. DOI : 10.1002/tox.22194

Finnell, R. H. (1999). Teratology: General Considerations and Principles. J Allergy Clin Immunol 1999; 103;S337-42

B. Harborne, Phytochemical Method, Chapman and Hall Ltd, London (1984)

George, A.J. Legal Status and Toxicity of Saponins. Food Cosmet. Toxicol. 1965, 3, 85–89. [CrossRef] 17.Pietta, P.G. Flavonoids as antioxidants.J. Nat. Prod. 2000, 63, 1035–1042. [CrossRef] [PubMed

Procházkováa, D.; Boušová, I.; Wilhelmováa, N. Antioxidant and prooxidant properties of flavonoids. Fitoterapia 2011, 82, 513–523. [CrossRef] [PubMed]

Organisation for Economic Co-operation and Development (2001) OECD Guideline for the Testing of Chemicals: Acute Oral Toxicity -Acute Toxic Class Method. 14 p.

Almanca CCJ, Saldanha SV, Sousa DR, Trivilin LO, Nunes LC, Porfírio LC, Marinho BG. Toxicological evaluation of acute and sub-chronic ingestion of hydroalcoholic extract of Solanum cernuum Vell. in mice. J Ethnopharmacol.2011;138(2):508-12.

Mukinda JT, Eagkes FK. Acute and sub-acute oral toxicity profile of the aque-ous extract of Polygala fruicosa in female mice and rats. J Ethnopharmacol. 2010;128(1):236-40.

Published

2020-12-31

How to Cite

Simanjuntak, N. J. P. ., Rosidah, & Yuandani. (2020). Evaluation of Acute Toxicity of Ethanol Extract of Pirdot Leaf (Saurauia vulcani Korth.) in Rats. Indonesian Journal of Pharmaceutical and Clinical Research, 3(2), 13-18. https://doi.org/10.32734/idjpcr.v3i2.4303

Most read articles by the same author(s)