Immunomodulator Activity of Puguntano (Picria fel-terrae Lour.) Extract in White Male Mice By Carbon Clearance Method
Keywords:Picria fel-terrae Lour, immunodulator, carbon clearance
Poguntano Herb Plants (Picria fel-terrae Lour.) contains flavonoids compounds that have been potentially to developed into immunomodulators. The purpose of this study was to determine the immunomodulatory activity of Poguntano herb extract (Picria fel-terrae Lour.), with the method of carbon clearance. n-hexane extract puguntano herb(ENHHP), extract of ethyl acetate of Poguntano herb (EEAHP), and ethanol extract of puguntano herb(EEHP) Poguntano multistage results in the test of immunomodulatory activity with a method of carbon clearance. Results showed that the EEHP 200 mg/kg BB showed a strong immunostimulation effect, EEAHP 200 mg/kg BB showed strong immunostimulation effect, ENHP 200 mg/kg BB demonstrated strong immunostimulation effect. It is thought that extract N-hexane herb Poguntano (ENHHP), extracts of ethyl herb acetate Poguntano (EEAHP), and herb ethanol extract Poguntano (EEHP) Poguntano herb may increase phagocytosis activity. Extract of ethyl acetate Poguntano herb 200 mg/kg bw (EEAHP) has the best phagocytosis activity with a value of 2.376 which indicates there is a significant difference (P < 0.05) with another treatment group.
. K. Abbas, A. H. Lichtman, and S. Pillai, “Preface,” Cellular and Molecular Immunology, p. v, 2010.
. N. Suciu-Foca, “Human Immunology,” Human Immunology, vol. 52, no. 1, p. iii, Jan. 1997.
. R. W. Blamey, “Basic and clinical immunology. Edited H. H. Fudenberg, D. P. Stites, J. L. Caldwell and J. V. Wells. Second edition. 255 × 180 mm. Pp. 758. Illustrated. 1978. California: Lange. $14.50,” British Journal of Surgery, vol. 66, no. 5, pp. 372–372, May 1979.
. N. Owen, “An herbal therapeutic approach to food intolerance and immune dysfunction: An illustrative case history,” Journal of Herbal Medicine, vol. 1, no. 2, pp. 53–63, Nov. 2011.
. M. Pittler, “Immunomodulatory Agents from Plants,” Focus on Alternative and Complementary Therapies, vol. 5, no. 3, pp. 233–233, Jun. 2010.
. U. Harahap, P. Patilaya, Marianne, S. Yuliasmi, D.I Husori, BE. Prasetyo, B. E., et al. “Profil Fitokimia Ekstrak Etanol Daun Poguntano (Curanga fel-terrae (Merr.) Lour.) yang berpotensi sebagai antiasma”. Seminar Nasional Sains dan Teknologi V, pp.422- 426, 2013.
. E. Middleton and C. Kandaswami, “Plant Flavonoid Modulation of Immune and Inflammatory Cell Functions,” Nutrition and Immunology, pp. 239–266, 1993.
. S. Katiyar, “Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin,” International Journal of Oncology, Dec. 2002.
. RI. Depkes , Herbal Pharmacopoeia Ed I, Jakarta: Departemen Kesehatan Republik Indonesia, pp. 164-175, 2013.
. Shabudeen, “Phytochemical Qualitative Analysis and Immunomodulator Activity of Agaricus bisporous Ethanol Extract by Carbon Clearance Technique,” Biochemistry & Pharmacology: Open Access, vol. 04, no. 02, 2015.
. M. Roseno, Y. Sudaryat, and W. Widyastiwi, “Uji Aktivitas Immunomodulator Berbagai Tanaman Famili Piperaceae pada Mencit Galur Balb/C dengan Metode Carbon Clearance,” Jurnal Ilmu Kefarmasian Indonesia, vol. 17, no. 2, p. 255, 2019.
. C.Kala, SS. Ali S.S and NA.Khan, Immunostimulatory Potential of n Butanolic Fraction of Hydroalcoholic Extract of Costus Speciosus Koen Rhizome. International Journal of Pharmacyand Pharmaceutical Sciences; vol.6, no. 7, pp. 2886-2892.2015
. KG. Baratawidjaja, and I Rengganis, Imunologi Dasar. Edisi ke-10. Jakarta: Balai Penerbit Fakultas Kedokteran Universitas Indonesia. Halaman 96, 110. 2012
. D. Bendjeddou, K. Lalaoui, and D. Satta, “Immunostimulating activity of the hot water-soluble polysaccharide extracts of Anacyclus pyrethrum, Alpinia galanga and Citrullus colocynthis,” Journal of Ethnopharmacology, vol. 88, no. 2–3, pp. 155–160, Oct. 2003.
. H. Wagner, H. Immunomodulatory Agents from Plants. Berlin : Birkhauser Verlag, p 15, 1999
. G.-G. Deng, W. Wei, X.-W. Yang, Y.-B. Zhang, W. Xu, N.-B. Gong, Y. Lü, and F.-F. Wang, “New coumarins from the roots of Angelica dahurica var. formosana cv. Chuanbaizhi and their inhibition on NO production in LPS-activated RAW264.7 cells,” Fitoterapia, vol. 101, pp. 194–200, Mar. 2015.
. K. S. Eom, H.-J. Kim, H.-S. So, R. Park, and T. Y. Kim, “Berberine-Induced Apoptosis in Human Glioblastoma T98G Cells Is Mediated by Endoplasmic Reticulum Stress Accompanying Reactive Oxygen Species and Mitochondrial Dysfunction,” Biological & Pharmaceutical Bulletin, vol. 33, no. 10, pp. 1644–1649, 2010.
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