Growth Response of Potato (Solanum tuberosum L.) to Application of Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) by In Vitro

Abstract

Potatoes are one of the important food crops in the world and a source of carbohydrates. However, providing seeds is still a problem due to their vulnerability to pests and disease. Technique tissue culture is an alternative for propagating Granola potato varieties. Influence giving concentrations of Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) on growth potato (Solanum tuberosum L.) in vitro. This research was carried out in the Tissue Culture Laboratory Alifa Agricultural Research Center (ALIFA-ARC) Medan. This research was conducted in February until May 2024. This research used a Completely Randomized Design (CRD) method with 2 factors treatment. The first factor is the concentration of Indole Acetic Acid (I): IO (0 mg/l), I1 (0,2 mg/l), I2 (0,3 mg/l). The second factor is the concentration of Benzyl Amino Purine (B): B0 (0 mg/l), B1 (2 mg/l), B2 (3 mg/l). Parameter observed in this study included, percentage of live explants, percentage of dead explants, time to emergence shoots, number of shoots, length of axillary shoots, number of roots, root length, and callus weight. The result shows that the combination of IAA and BAP has a significant effect on all observation parameters. The best treatment for shoot growth was found in I0B2, root growth in treatment I2B0, and callus growth on I2B2.