Kombinasi 2,4 D Dan BAP Untuk Induksi Kalus Embriogenik Beberapa Varietas Kedelai (Glycine Max (L.) Merrill) Pada Kondisi Hipoksia Secara In Vitro
The Combination 2,4-D and BAP to Induce Embryogenic Callus from Some Varieties of Soybean (Glycine max (L.) Merrill) on Hypoxic and In Vitro Condition
DOI:
https://doi.org/10.32734/joa.v6i1.2544Keywords:
2,4-D, BAP, embryogenic callus, hypoxic, soybean, hipoksia, kalus embriogenik, kedelaiAbstract
The purpose of this research was to determinate the growth response of embryogenic callus from
some varieties of soybean (Glycine max (L.) Merrill) on hypoxic and in vitro condition.The research
was conductedat The Tissue Culture Laboratory of Agriculture FacultyinUniversity of Sumatera
Utara on April until December 2016. This research had two stages, induction of embryogenic callus
and analysis metabolism of callus after hypoxic condition with t -test. This analysis used factorial
Completely Randomized Design with two factors. The first factors were varieties of soybean and
the second factors werecombination of Plant Growth Regulator (PGR)(5 mg/l 2,4-D + 1 mg/l BAP,
10 mg/l 2,4-D + 1,5 mg/l BAP, and 15 mg/l 2,4-D + 2 mg/l BAP). The result showed t he varieties,
combination of PGR, and interaction of varieties and PGR give significant effect to weight callus.
The result of T-test showed that in hypoxic condition, POD enzyme exercise on Gepak Kuning’s
callus in 5 mg/l 2,4-D + 1 mg/l BAP was different before and after hypoxic condition.
Penelitian ini bertujuan untuk mengetahui respon pertumbuhan dan perkembangan kalus emberiogenik pada berbagai varietas kedelai dengan pemberian kombinasi 2,4-D dan BAP pada kondisi hipoksia secara in vitro. Penelitian dilaksanakan di Laboratorium Kultur Ja ringan Tanaman Universitas Sumatera Utara, pada bulan April sampai Desember 2016. Metode penelitian dilakukan dengan 2 tahapan yaitu induksi kalus embriogenik dan uji-t terhadap analisis aktivitas metabolit kalus setelah penggenangan. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial dengan dua faktor perlakuan. Faktor pertama adalah varietas yang diuji (Baluran, Gepak Kuning, dan Grobogan). Faktor kedua adalah kombinasi Zat Pengatur Tumbuh (5 mg/l 2,4-D + 1 mg/l BAP, 10 mg/l 2,4-D + 1,5 mg/l BAP, dan 15 mg/l 2,4-D + 2 mg/l BAP). Hasil analisis data menunjukkan bahwa varietas, kombinasi ZPT, dan interaksi vaerietas dan ZPT berpengaruh nyata terhadap induksi kalus pada peubah amatan bobot kalus. Berdasarkan analisis uji-t, aktivitas enzim POD pada varietas Gepak Kuning yang diinduksi media 5 mg/l 2,4-D + 1 mg/l BAP memberikan respon yang berbeda nyata sebelum dan sesudah penggenangan.
Downloads
Published
Issue
Section
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Jurnal Online Agroekoteknologi as well as TALENTA Publisher Universitas Sumatera Utara as the publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all forms and media. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, will be allowed only with written permission from Jurnal Online Agroekoteknologi.
The Copyright Transfer Form can be downloaded here.
The copyright form should be signed originally and sent to the Editorial Office in the form of original mail or scanned document.