Keberhasilan Terbentuknya Tunas Mikro Anggrek (Cattleya trianae Lindl & Rchb.fil.) Dalam Beberapa Komposisi Medium
The achievement of shaped micro shoots orchid (Cattleya trianae Lindl & Rchb.fil.) in some mediums composition
DOI:
https://doi.org/10.32734/ja.v6i1.2556Keywords:
Cattleya, medium composition, multiplication, shoot, type of medium, bentuk media, jenis komposisi media, multiplikasi, tunasAbstract
The research aims to find the most suitable multiplication medium for orchid propagation. The
research was conducted in Tissue Culture Laboratory U PT. Seeds Horticulture, Johor, Medan,
North of Sumatera, Indonesia, from September 2016 to January 2017. Th e study used completely
randomized design with two factors; types of medium, consist of two levels, B1: liquid medium and
B2: solid medium, and six medium composition, J1: MS + BAP 1 ppm + coconut water 150 ml/l;
J2: ¾ MS + BAP 1 ppm + coconut water 150 ml/l; J3:½ MS+ BAP 1 ppm + coconut water
150 ml/l; J4:VW+ BAP 1ppm + coconut water 150 ml/l; J5: Nitsch & Nitsch + BAP 1 ppm +
coconut water 150 ml/l; J6: ½ Nitsch & Nitsch + BAP 1ppm + coconut water.The results showed
that solid medium shows the best medium multiplication of orchid. Medium ½ NN + BAP 1 ppm +
coconut water 150 ml/l; M edia ¾ MS + BAP 1 ppm + coconut water 150ml/L are the most of
medium shoot induction. The interaction between solid medium and combination medium NN +
BAP 1 ppm + coconut water 150 ml/l gave the shoot and root prolongation of Cattleya .
Penelitian ini bertujuan untuk mendapatkan media multiplikasi yang terbaik dan efisien dari segi penggunaan bahan kimia untuk produksi bibit anggrek Cattleya trianae Lindl & Rchb.fil. melalui kultur in vitro. Penelitian ini dilakukan di Laboratorium Kultur Jaringan UPT. Balai Benih Induk Hortikultura Gedung Johor Medan, Sumatera Utara, Indonesia, pada September 2016 sampai dengan Januari 2017. Penelitian ini menggunakan rancangan acak l engkap dengan dua faktor, yaitu: bentuk media, terdiri dari dua tingkatan, B1: media cair dan B2: media padat, dan jenis komposisi media terdiri dari enam komposisi, J1: MS + BAP 1 ppm + air kelapa150 ml/l; J2: ¾ MS + BAP 1 pm + air kelapa 150 ml/l; J3: ½ MS+ BAP 1 ppm + air kelapa 150 ml/l; J4 : VW+ BAP 1ppm + air kelapa 150 ml/l; J5: Nitsch & Nitsch + BAP 1 ppm + air kelapa 150 ml/l; J6: ½ Nitsch & Nitsch + BAP 1ppm + air kelapa 150 ml/l. Hasil penelitian menunjukkan bahwa media padat menunjukkan media multiplikasi anggrek terbaik. Media ½ NN + BAP 1 ppm + air kelapa 150 ml/l merupakan media terbaik untuk munculnya tunas tercepat dan jumlah tunas terbanyak. Interaksi bentuk media padat dengan jenis komposisi mediaNN + BAP 1 ppm + air kelapa 150 ml/l memberi pertambahan panjang tunas dan pertambahan panjang akar pada anggrek Cattleya.
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