Keragaman Genetik Populasi Elaeis oleifera Dan Populasi Elaeis oleifera x Elaeis guineensis Pada Koleksi Plasma Nutfah PPKS Berdasarkan Marka Simple Sequence Repeats
Genetic Diversity of E. oleifera Populations and E. oleifera x E. guineensis Populations of IOPRI’S Oil Palm Germplasm Based on Simple Sequence Repeat Markers.
DOI:
https://doi.org/10.32734/joa.v6i2.2597Keywords:
E. oleifera, Genetic Diversity, Genetic Relationship, Interspecific Hybrids, Simple Squence Repeats, Hibrida Interspesifik, Kekerabatan Genetik, Keragaman GenetikAbstract
The results showed that the total of alleles were found 57 alleles with ranged from 4 to 10 alleles
per primer with Polymorphic Information Content (PIC) ranged from 0.55 to 0.82. The number of
alleles in 4 populations of E. oleifera Suriname, E. oleifera Brazil and their interspecific hybrids
(BC1 Suriname and BC1 Brazil) ranged from 2.33 to 3.67 per population with the percentage of
polymorphic loci ranged from 36% - 100%. The best primer in this study was mEgCIR3555
(10 alleles per primer) with PIC values of 0.82 and mEgCIR0782 (8 alleles per primer) with PIC
values of 0.81. Dendogram analysis spread 29 samples into three main groups with a genetic
distance ranged from 0.04 to 0.98 and molecular diversity can be explained using simple sequence
repeats marker was 57.29%.
Hasil penelitian menunjukkan bahwa dari kesembilan marka SSR mampu mendeteksi total alel sebanyak 57 alel dengan kisaran 4 - 10 alel per primer dengan nilai Polymorphic Information Content (PIC) diperoleh berkisar antara 0.55 - 0.82. Jumlah alel pada 4 berkisar antara 2.3 – 3.67 per populasi dengan nilai persentase lokus polimorfik diperoleh berkisar antara 36% - 100%, primer yang terbaik pada penelitian ini yaitu mEgCIR3555 (10 alel per primer) dengan nilai PIC sebesar 0.82 dan mEgCIR0782 (8 alel per primer) dengan nilai PIC sebesar 0.81. Analisis dendogram keempat populasi yaitu E. oleifera Suriname, E. oleifera Brazil dengan hibrida interspesifiknya (BC1 Suriname dan BC1 Brazil) terbagi ke dalam 3 group besar dengan jarak genetik b erkisar antara 0.04 - 0.98, keragaman genetik yang dapat dijelaskan oleh marka SSR pada penelitian ini sesebar 57.29%.
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