Effect of Innoculation Duration and Number of Hole Container to Cotesia flavipes (Hymenoptera; Branconidae) Propogation in Laboratory

Abstract

This research was aimed to study the number of container holes and the inoculation period of  C. flavipes. This research was conducted in the sugarcane Laboratory of Research and Development Sei Semayang Binjai, Sumatera Utara from November 2017 to January 2018. A complete randomized design with two factors and three replications was used. The first factor was the number of holes in a container (1 hole d=10 cm, 1 hole d=2.5 cm, 4 holes d=2.5 cm, 7 holes d=2.5 cm and the second factor was incubation period (10, 20, dan 30 minutes). The result showed that the number of holes in each container and the inoculation period was significantly affecting the parasitation period, parasitation presence, number of images, and sex ratio of C. flavipes. The highest percentage (33.68%) on the number of container 1 hole d=10 cm while the lowest (7.38%) was in 1 hole d=2.5 cm. In terms of the incubation period, the highest (24.14%) parasitation percentage was in 10 minutes and the lowest (15.81%) was in 30 minutes. The highest number of imago (319.92 C. flavipes) based on inoculation treatment = 10 minutes, while the lowest (207.25 C. flavipes) = 20 minutes. The highest male (207.78 C. flavipes) was in 1 hole d=10 cm and the lowest (48.89 C. flavipes) in 1 hole d=2.5cm. The highest female (219.44 C. flavipes) was in 1 hole d=10 cm and the lowest (54.00 C. flavipes) in 1 hole d=2.5 cm. The highest (168.00 C. flavipes) inoculation period = 10 minutes while the lowest (107.5   C. flavipes) was in inoculation = 20 minutes. The sex ratio male : female = 1 : 1.