Effects of Gamma Ray Irradiation to Induce Genetic Variability of Teak Planlets (Tectona grandis Linn. F.)
DOI:
https://doi.org/10.32734/jsi.v5i01.6166Keywords:
Genetic Variation, Mutant, Radiosensitivity, RAPD, Teak (Tectona grandis)Abstract
Teak planlets (Tectona grandis) of Salomon clones were irradiated by gamma rays to induce genetic variability for growth improvement. The objectives of this research were to analyze the radiosensitivity of T. grandis Salomon clones by gamma ray irradiation and to analyze the genetic variations using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD). For those purposes, teak planlets were irradiated using gamma rays at 5 different dosages, those were 0 Gy, 10 Gy, 20 Gy, 30 Gy, and 40 Gy. Lethal doses50 (LD50) and reduced doses50 (RD50) were obtained using Curve-fit Analysis. Furthermore, the irradiated planlets were subcultured on medium MS + 0.1 kinetin (M1V0) the surviving M1V0 plantlets were multiplied to MS + 0.1 kinetin, so it is obtained the M1V1 generation. Genetic variation of the mutant was molecularly analyzed using RAPD methods and the variability was calculated using Analysis of Molecular Variance (AMOVA). Results of this research showed that lethal doses50 (LD50) and reduced doses50 (RD50) values were obtained at 24.5 Gy and 7.85 Gy, respectively. It includes genomes of small size and a small number of genomes. The genetic variation of mutant individuals in among and within the treatment of gamma irradiation was 16% and 84% according to AMOVA. It means that the dominant effect of genetic material caused genetic variation in mutant M1V1 generations. This dominance of genetic material could be caused by the response combination of tissue culture treatment and genetic factors that had the potency to be used as materials to select desired clones in the next stage.
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Akshatha, Chandrashekar, K. R, Somashekarappa, H. M., Souframanien, J. 2013. Effect of gamma irradiation on germination, growth, and biochemical parameters of Terminalia arjuna Roxb. Radiation and Environ 36(1): 38-44.
Amom, T., Tikendra, L., Apana, N., Goutam M., Sonia, P., Koijam, A. S., Potshangbam, A. M., Rahaman, H., Nongdam, P. (2020) Efficiency of RAPD, ISSR, iPBS, SCoT and phytochemical markers in the genetic relationship study of five native and economical important bamboos of North-East India. Phytochemistry 174: 112330.
Anandan, R., Prakash, M., Deenadhayalan, T., Nivetha, R., Kumar, N. S. 2018. Efficient invitro plant regeneration from cotyledon-derived callus cultures of Sesame (Sesamum indicum L.) and genetic analysis of true-to-type regenerants using RAPD and SSR markers. South African Journal of Botany 119: 244–251
Annisa, Hafzari, R., Setiawati, T., Irawan, B., Kusmoro, J. 2019. Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia. Folia Forestalia Polonica, Series A – Forestry 61 (4), 255–266. DOI: 10.2478/ffp-2019-0025
Antony, T., Anees, P. M. W., Kumar, V., Sangamithra, D., Philp, T, Santhoshkumar, A. V. 2015. Application of mercuric chloride and charcoal in micro-propagation of teak (Tectona grandis). Indian Journal of Tropical Biodiversity 23(2): 157-166.
Basha, M. H., Mehta, A. K., Gour, V. K., Kachare, S. 2015. Biological effects of gamma irradiation in oat (Avena sativa L.). Range Mgmt. & Agroforestry 36(1): 60-65.
Borzouei, A., Kafi, M., Khazaei, H., Naseriyan, B., Majdabadi, A. 2010. Effects of gamma radiation on germination and physiological aspects of wheat (Triticum aestivum L.) seedlings. Pak. J. Bot 4(2): 2281-2290.
Bhargava, Y. R., Khalatkar. 1985. Improve performance of tectona grandis seeeds with gamma irradiation [abstract]. International society for horticultural science 215. http://www.achaort.org.
Caro-Melgarejo, D.P., Estupián-Rincón, S.Y., Rache-Cardenal, L.Y., Pacheco-Maldonado, J.S. 2012. Effect of gamma rays on vegetative buds of Physalis peruviana L. Acta Agronómica 61(4): 277-285.
Chanchula, N., Taychasinpitak, T., Jala, A., Thanananta, T., Kikuchi, S. 2015. Radiosensitivity of in vitro cultured Torenia fournieri Lind. from Thailand by γ-ray irradiation. Int Trans J Eng Manag Sci Tech 6(4): 157-164.
Chaudhari, C., Jha, S. K., Dhaka, R. K., Parekh, V., Sankanur, M.S., Prajapat, P., Thakur, S. 2018. Genetic diversity analysis of teak in South Gujarat by RAPD marker. International Journal of Chemical Studies 6(6): 260-267.
Dotaniya, M.L., Meena, V. D., Lata, M., Meena, H. P. 2013. Teak plantation- a potential source of income generation. Popular Kheti 1(3): 61-63.
Forster B. P., Shu, Q. Y. 2012. Plant mutagenesis in crop improvement: basic terms and applications. In: Shu QY, Forster BP, Nakagawa H. (ed). Plant Mutation Breeding and Biotechnology. Italy: CAB International and FAO:9-21.
Galván, I., Bonisoli-Alquati, A., Jenkinson, S., Ghanem, G., Wakamatsu, K., Mousseau, T., Møller, A. 2014. Chronic exposure to low-dose radiation at Chernobyl favours adap tation to oxidative stress in birds. Funct. Ecol.28: 1387–1403.
Geras’kin, S., Evseeva, T., Oudalova, A. 2013. Effects of long-term chronic exposure to radionuclides in plant populations. J Environ Radioact 121: 22-32.
Hasbullah, N. A., Taha, R. M., Saleh, A., Mahmad, N. 2012. Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii. Hortic. Bras 30(2): 252-257.
Hwang, J. E., Jang, D., Lee, K. J., Ahn, J., Kim, S. H., Kang, S., Kim, D.S., Kim, J. 2016. Identification of gamma ray irradiation-induced mutations in membrane transport genes in a rice population by TILLING. Genes & Genetic Systems 91(5): 1-24.
Iglesias Andreu, L.G., Octavio Aguilar, P., Bello Bello, J. 2012. Current importance and potential use of low doses of gamma radiation in forest species. in: Adrovic F.(ed): Gamma Radiation. Mexico: In Tech Publishing.
Kadir, A. 2011. Effects gamma ray irradiation on plants patchouli shoot. J.Agrivigor 10(2): 177-127. [Indonesian]
Kollert, W., Cherubini, L. 2012. Planted Forests and Trees Working Paper Series Teak Resources And Market Assessment 2010 (Tectona grandis Linn. F.). Italy: FAO.
Kumar, S., Kumar, M., Yadav, H. K., Sharma, S., Kumar, S. 2017.Genetic diversity and population structure analysis of Chrysanthemum (Dendranthema grandiflora Tzvelev) germplasm based on RAPD marker. Journal of Environmental Biology 38: 457-464
Minhas, R. A., Khan, M. N., Awan, M. S., Ahmad, B., Bibi, S. S., Hanif, M., Mian, A.2018. RAPD based genetic diversity of endangered Himalaya gray langur
(semnopithecus ajax) population of Pakistan. Pakistan J. Zool 50(6): 2059-2071.
Minisi, A. F., El-Mahrouk, E., Rida, F. M., Nasr, M. 2013. Effect of gamma radiation on germination, growth characristics and marphological variation of Molluccella leavis L. American-Eurasian J Agric & Environ Sci 13(5): 696-704.
Modi, J. S., Tandel, M.B., Prajapati, V. M., Parekh, V. B., Ahir, B. R. 2018. Molecular variations in teak (Tectona grandis L. f.) clones. International Journal of Chemical Studies 6(5): 259-269.
Mosllem, S., Behbahani, M., Mousavi, E. S. 2012. Effect of gamma radiation on callus induction and regeneration of Rosa canina through in vitro culture. Trakia Journal of Sciences 11(2): 158-162.
Nadeem, M. A., Nawaz, M. A., Shahid, M. Q., Dogan, Y., Comertpay, G., Yildiz, M., Hatipoglu, R., Ahmad, F., Alsaleh, A., Labhane, N., Ozkan, H., Chung, G., Baloch, F. S. 2017. DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing. Biotechnology and Biotechnological Equipment, 32 (2), 261–285
Nurmansyah., Alghamdi, S. S., Migdadi, H. M., Farooq, M. 2018. Morphological and chromosomal abnormalities in gamma radiation-induced mutagenized Faba bean genotypes. International Journal of Radiation Biology 90(2): 174-185.
Ohri, D.., Kumar, A. 1986. Nuclear DNA amounts in some tropical hardwoods. Caryologia 9(3-4): 303-307.
[PP] Peraturan Pemerintah. 2007. Government regulation of Indonesia Number Republic 44 1995 about seedling. Jakarta [Indonesian].
Perum Perhutani 2013. Business and Core Process Structuring Annual Report 2013 [Indonesia].
RajaRamadoss, B., Ganesamurthy, K., Angappan, K., Gunasekaran, M. 2014. Mutagenic effectiveness and efficiency of gamma rays in sesame (Sesamum indicum L.). Global Journal of Molecular Sciences 9(1): 01-06.
Rastogi, J., Siddhant., Bubber P., Lal., Sharma, B. 2015. Somaclonal variation: a new dimension for Sugarcane improvement. GERF Bulletin of Biosciences 6(1): 5-10.
Rauf, S., Jaime, A., da Silva, T., Khan, A. A., Naveed, A. 2010. Consequences of plant breeding on genetic diversity. International Journal of Plant Breeding 4(1): 1-21.
Roshetko, J. M., Rohadi, D., Perdana, A., Sabastian, G., Nuryartono, N., Pramono, A. A., Widyani, N., Manalu, P., Fauzi, M.A., Sumardamto, P., Kusumowardhani, N. 2013. Teak agroforestry systems for livelihood enhancement, industrial timber production, and environmental rehabilitation. Forests, Trees and Livelihoods 22(2): 241–256.
Sadono, R. 2014. Determining growing space of Perhutani’s teak plus plantation in Madiun, Saradan and Ngawi Forest District, East Java, Indonesia. Australian Journal of Basic and Applied Sciences 8(10): 427-437.
Sontikun, Y., Chanprame, S., Srinives, P., Chanprame, S. 2013. Optimization of transient β-glucuronidase (gus) gene expression in teak (Tectona grandis L.f.) by Agrobacterium tumefaciens-mediated transformation system. J ISSAAS 19(2): 49-57.
Sreedhar., Anurag, C., Aparna, M. M., Pavan, D. K., Singal, R. K., Venu-Babu, P. 2013. Influence of γ- radiation stress on scavenging enzyme activity and cell ultra structure in groundnut (Arachis hypogaea L.). Pelagia Research Library, 4(2): 35-44.
Thakur, R.C., Ishii, K. 2012. Detection and fingerprinting of narrow-leaf mutants in micro-propagated hybrid poplar (Populus sieboldii x P grandidentata) using random amplified polymorphic DNA. International Journal of Farm Sciences, 2(1): 79-84.
Thuy, M. T P., Ha, T. T. T., Quang, T. H. 2020. Analysis of genetic diversity in Pa Co pine (Pinus kwangtungensis Chun ex Tsiang) using RAPD and ISSR markers. Biotechnologyvol 62(1): 62-68.
Ugorji, O. U., Ikpeme, E.V., Obu, J. A., Ekpenyong, E. D. 2012. Assessing the mutagenic effects of gamma irradiation on Cajanus cajan (L.) Huth and Vigna unguiculata (L.) Walp landraces using morphological markers. Comunicata Scientiae 3(4): 271-281.
Widyatmoko, A. Y. P. B. C., Rimbawanto, A., Chasani, A. R. 2013. Genetic relationship among teak (Tectona grandis, Linn.f) populations based on RAPD (Random Amplifieds Polymorphic DNA) markers. Jurnal Pemuliaan Tanaman Hutan 7(3): 151-166. [Indonesian]
Walpole, R. E. 1982. Introduction to Statistics Third Edition. Jakarta. Gramedia Pustaka Utama [Indonesian].
Witjaksono, Litz, R. E. 2004. Effect of gamma irradiation on embryogenic Avocado cultures and somatic embryo development. Plant Cell, Tissue and Organ Culture 77: 139–14.
Yasmin, K., Arulbalachandran, D. 2016. Effect of gamma rays on morphological and quantitative traits of black gram (Vigna mungo (L.) Hepper) in M1 generation. Int. J. Cur. Tr. Res 4(2): 5-15.
Yaycili, O., AlikamanoÄŸlu, S. 2012. Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis. Turk J Biol 36: 405-412
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