The Activity Of Cellulose Enzyme From Indigenous Bacteria "Bacillus Sp YLB1" As Bioactivator

Authors

  • Yunilas Animal Production Study Program, Universitas Sumatera Utara, Padang Bulan, Medan 20155, Indonesia
  • Lili Warly Department of Animal Nutrition, Faculty of Animal Husbandry, University of Andalas, Indonesia
  • Yetti Marli Department of Animal Nutrition, Faculty of Animal Husbandry, University of Andalas, Indonesia
  • Irsan Riyanto Department of Animal Nutrition, Faculty of Animal Husbandry, University of Andalas, Indonesia

DOI:

https://doi.org/10.32734/jpi.v7i2.2143

Keywords:

cellulase enzyme, indigenous bacteria, substrate

Abstract

This study aims to test the activity of cellulase enzyme (CMCase) from
indigenous bacteria "Bacillus Sp YLB1" as bioactivator of palm oil based feed. The
treatment consists of various combinations of substart, namely: P1 = 80% palm leaves +
10% palm kernel cake + 10% sludge; P2 = 60% palm leaves + 20% palm kernel cake +
20% sludge and P3 = 60% palm leaves + 30% palm kernel cake
+ 30% sludge. The parameters observed were cellulase enzyme activity (CMCase) on
various combinations of palm oil waste and fermentation time. The results showed that
cellulase enzyme (CMCase) production of Bacillus sp YLB1 bacteria during fermentation
fluctuated ie 0.143 Units / ml; 0.372 Units / ml; 0.588 Units / ml; 1.013 Units / ml; 0.906
Units / ml; 1,065 Units / ml and 1,198 Units / ml.The activity of cellulase enzyme
(CMCase) on substrate P1 is lower than P2 and P3. As conclutions that cellulase enzyme
activity of Bacillus sp YLB1 influenced by the combination of substrate and fermentation
time. The P3 fermented with Bacillus sp YLB1 for 7 days gave a more optimal result than
the other treatments

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Published

2019-06-24

Issue

Vol. 7 No. 2 (2019): Jurnal Peternakan Integratif

Section

Articles

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