Antibacterial Test of Dadih Against Pathogenic Bacteria and Its Effects on Native Chicken Immunity Organs
DOI:
https://doi.org/10.32734/injar.v7i2.15898Keywords:
antibiotic, bursa fabrisius, dadih, native chicken, spleen, thymusAbstract
Dadih contains lactic acid bacteria that are antimicrobial so that it can be utilized to maintain the health of poultry such as native chickens. This study aims to prove the ability of dadih to suppress the growth of pathogenic bacteria, namely Staphylococcus aureus, Escherichia coli and Salmonella. This study will also try to find out invivo effect of dadih on the organ immunity of native chicken infected with Salmonella. Antimicrobial research uses disc diffusion method with antimicrobial zone parameter. The invivo study was conducted using a completely randomized design with 4 treatments and five replications where the parameter was the development of immunity organs namely thymus, bursa fabrisius and spleen. The results showed that the antimicrobial test of dadih against Staphylococcus aureus, Escherichia coli and Salmonella caused a zone of inhibition of 7.84, 6.63 and 8.34 mm. Dadih treatment significantly affected the percentage of thymus, bursa fabrisius and spleen. As conclusion that dadih is able to suppress the growth of Staphylococcus aureus, Escherichia coli and Salmonella at medium strength with a range of inhibition zone between 6.63-8.34. Dadih is also able to maintain the health of native chicken.
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J. Jerab, W. Jansen, J. Blackwell, J. Van Hout, A. Palzer, S. Lister, I. Chantziaras, J. Dewulf, and N. De Briyne, “Real-world data on antibiotic group treatment in European livestock: drivers, conditions, and alternatives,” Antibiotics, vol. 11, No. 8, p. 1046, 2022.
G. Busch, B. Kassas, M. A. Palma, and A. Risius, “Perceptions of antibiotic use in livestock farming in Germany, Italy and the United States,” Livestock Science, vol. 241, p. 104251, 2020.
M. Casewell, C. Eriis, E. Marco, P, McMullin, and I. Phillips, “The European ban on growth-promoting antibiotics and emerging consequences for human and animal health,” Journal of Antimicrobial Chemotherapy, vol. 52, no. 2, pp. 159-161, 2003.
S. D. Yanti, E. Mirwandhono, and N. Ginting, “Nutrition Content of Murrah Buffalo's Milk Curd By Using Lime Juice (Citrus aurantifolia),” Jurnal Peternakan Integratif, vol. 10, no. 2, pp. 78-84.
C. H. Sirait, N. Cahyadi, T. Panggabean, and I. G. Putu, “Identifikasi dan Pembiakan Kultur Bakteri Pengolah Dadih Laporan Penelitian Balai Penelitian Ternak, Ciawi, Bogor, 1995.
S. K. Tiwari, “Bacteriocin-producing probiotic lactic acid bacteria in controlling dysbiosis of the gut microbiota,” Frontiers in Cellular and Infection Microbiology, vol. 12, p. 851140, 2022.
O. Gillor, A. Etzion and M. A. Riley, “The dual role of bacteriocins as anti-and probiotics,” Applied Microbiology and Biotechnology, vol. 81, pp. 591-606, 2008.
S. C. Yang, C. H. Lin, C. T. Sung, and J. Y. Fang, “Antibacterial activities of bacteriocins: application in foods and pharmaceuticals,” Frontiers in Microbiology, vol. 5, p. 91530, 2014.
W. W. Davis and T. R. Stout, “Disc plate method of microbiological antibiotic assay: I. Factors influencing variability and error,” Applied Microbiology, vol. 22, No. 4, pp. 659-665, 1971.
M. Dec, A. Puchalski, A. Nowaczek, and A. Wernicki, “Antimicrobial activity of Lactobacillus strains of chicken origin against bacterial pathogens,” Int Microbiol, vol. 19, No. 1, pp. 57-67, 2016.
N. M. Nabil, M. M. Tawakol, and H. M. Hassan, “ssing the impact of bacteriophages in the treatment of Salmonella in broiler chickens,” Intection Ecology and Epidemiolohy, vol. 8, no. 1, p. 1539056, 2023.
S. Shaji, R. K. Selvaraj, and R. Shanmugasundaran, “.Sukhoom, "Enhancement of rice growth and yield in actual acid sulfate soils by potent acid-resistant Rhodopseudomonas palustris strains for producing safe rice," Plant Soil, vol. 11, no. 11, p. 2814.
C. M. Logue, Y. Wannemuehler, C. Doetkott, and N. L. Barbieri, “Comparative analysis of phylogenetic assignment of human and avian ExPEC and fecal commensal Escherichia coli using the (previous and revised) Clermont phylogenetic typing methods and its impact on avian pathogenic Escherichia coli (APEC) classification,” Frotiers in Microbiology, vol. 8, p. 248958, 2017.
L. S. M. Kabir, “Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns,” Internasional Journal of Environmental Research and Public Health, vol. 7, no. 1, pp. 89-114, 2010.
W. A. A. El-Ghany, “Staphylococcus aureus in poultry, with special emphasis on methicillin-resistant strain infection: a comprehensive review from one health perspective,” Internasional Journal of One Health, vol. 7, no. 2, pp. 257-267, 2021.
A. Aydin, M. Sudagidan, and K. Muratoglu, “Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey,” Internasional Journal of Food Microbiology, vol. 148, no. 2, pp. 99-106, 2011.
J. R. Fitzgerald, “Livestock-associated Staphylococcus aureus: origin, evolution and public health threat,” Trends in Microbiology, vol. 20, no. 4, pp. 192-198, 2012.
N. S. Jamilah and L. D. Mahfudz, “Performa produksi dan ketahanan tubuh broiler yang diberi pakan step down dengan penambahan asam sitrat sebagai acidifier,” JITV, vol. 18, no. 4, pp. 251-257, 2013.
W. Elisa, E. Widiastuti, and T. A. Sarjana, “Bobot relatif organ limfoid dan usus halus ayam broiler yang disuplementasi probiotik Bacillus plus,” Prosiding Seminar Nasional Teknologi Agribisnis Peternakan (STAP), vol. 5, pp. 297-301, 2017.
S. Umar and Y. Hulu, “Effect of Addition Sinbiotic Isolate Pediococcus pentosaceus Strain N6 and Cassava Peel on Immunity Organs and Total Microbes in the Small Intestine of Super Native Chicken,” IOP Conference Series: Earth and Environmental Science, vol. 1286, no. 1, p. 012033, 2023.
L. Wiranto, S. Sumarmih, and B. Sulistiyanto, “Bobot Relatif Organ Imun Ayam Broiler Dengan Metode Pemberian Probiotik Yang Berbeda,” Prosiding Seminar Nasional Teknologi Agribisnis Peternakan (STAP), vol. 7, pp. 690-698, 2020.
K. A. Pike, E. Baig, and M. J. Ratcliffe, “The avian B‐cell receptor complex: distinct roles of Igα and Igβ in B‐cell development,” Immunological reviews, vol. 197, no. 1, pp.10-25, 2004.
A. A. Shehata, S. Yalcun, J. D. Latorre, S. Basiouni, Y. A. Attia, A. A. El-Wahab, C. Visscher, R. H. El-Seedi, C. Huber, M. H. Hafez, W. Eisenreich, and G. Tellez-Isaias, “Probiotics, prebiotics, and phytogenic substances for optimizing gut health in poultry,” Microorganisms, vol. 10, no. 2, p. 395, 2022.
S. Naseem, S. U. Rahman, M. Shafee, A. A. Sheikh, and A. Khan, “Immunomodulatory and growth-promoting effect of a probiotic supplemented in the feed of broiler chicks vaccinated against infectious bursal disease,” Brazilian Journal of Poultry Science, vol. 14, pp. 109-113, 2012.
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