Isolation and Identification of Cellulolytic and Lignolytic Bacteria from the Gut Oryctes rhinoceros L. Larvae Decomposition of Oil Palm Empty Fruit Bunches
DOI:
https://doi.org/10.32734/injar.v1i2.314Keywords:
cellulolytic index, lignolytic index, oil palm empty fruit bunches, O. rhinoceros larvaeAbstract
One of the organisms that helps in decomposition of oil palm empty fruit bunches is Oryctes rhinoceros L. larvae. This is because of in the gut of the larvae there are many cellulolytic bacteria and lignoliytic. The process of accelerating the decomposition of oil palm empty fruit bunches into compost can be done by optimizing the work of the bacteria. The aim of this research is to obtain cellulolytic and lignolytic bacteria from larvae O. rhinoceros L.. The research succeeded to isolate 24 isolates of cellulolytic bacteria and lignolytic bacterial isolates from the gut of O. rhinoceros larvae. Based on qualitative test, 9 isolate bacteria produced cellulolytic index above 2. The highest cellulolytic index was generated by ORL19 isolate, while the lignolytic index obtained ORL6 isolate. Based on macroscopic and microscopic identification of bacteria, biochemical and physiological tests, it was found ORL 6 belongs to the genus Bacillus sp. while the ORL 19 belongs to the genus Citrobacter sp..
Downloads
References
Y. Fauzi, Y. E. Widiyastuti, I. Satyawibawa, and R. H. Paeru, Kelapa Sawit: Budidaya, Pemanfaatan Hasil dan Limbah, Analisis Usaha dan Pemasaran. Jakarta, Penebar Swadaya, 2005.
A. Sunaryo, M. Anggito, and M. Sumringahgesit, “Hydrolysis of a lignocellulosic materials oil palm empty fruit bunch for ethanol production,” Biomaterial Technology, vol. 803, pp.1-11. 2013.
D. P. Dewanti, “Potensi Selulosa dari Limbah Tandan Kosong Kelapa Sawit untuk Bahan Baku Bioplastik Ramah Lingkungan,” Jurnal Teknologi Lingkungan, vol. 19, no. 1, pp.81-88, 2018.
W. Hidayat, “Isolasi dan skrining bakteri cellulolytic dari saluran pencernaan larva O. rhinoceros (Coleoptera: Scarabaeidae),” Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Sebelas Maret, Surakarta, 2013.
T. C. dos Santos, D. P. P. Gomes, R. C. F. Bonomo, and M. Franco, “Optimisation of solid state fermentation of potato peel for the production of cellulolytic enzyme,” Food Chemistry, vol. 133, pp. 1299-1304, 2012.
A. S. Baharuddin et al., “Isolation and characterization of thermophilic cellulase-producing bacteria from empty bunches-palm oil mill effluent compost,” Journal of Applied Science, vol. 7, no. 1, pp.56-62. 2010.
S. L. A. Sari et al., “Cellulolytic and hemicellulolytic bacteria from the gut of O. rhinoceros larvae,” Jurnal Biodiversitas, vol. 17, no. 1, pp. 78-83, 2016.
L. Bandounas, N. J. P. Wierckx., J. H. de Winde, and H. J. Ruijssenaars, “Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential,” Journal of BMC Biotechnology, vol. 11, no. 94, 2011.
T. I. Sunatmo, Eksperimen Mikrobiologi dalam Laboratorium. Jakarta, Ardy Agency. 2009.
E. Chasanah, I. R. Dini, and N. R. Mubarik, “Karakterisasi enzim selulase PMP 0126Y dari limbah pengolahan agar,” Jurnal Pengolahan dan Bioteknologi Perikanan, vol. 8, no. 2, pp.103-114, 2013
A. J. Kader and O. Omar, “Isolation of cellulolytic fungi from Sayap Kinabalu Park, Sabah. Serawak,” Journal Biodiversity Bio-Conserv (ARBEC), pp.1-6, 1998.
H. T. Prakoso, H. Widiastuti., Suharyanto, and Siswanto, “Eksplorasi dan karakterisasi bakteri aerob lignolytic serta aplikasinya untuk pengomposan tandan kosong kelapa sawit,” Jurnal Menara Perkebunan, vol. 82, no. 1, pp.15-24, 2014.
J. G. Cappucino and N. Sherman, Microbiology: A Laboratory Manual. Wesley: Addison, 1983.
S. T. Pratiwi, Mikrobiologi Farmasi. Jakarta, Erlangga, 2008.
M. Lamid, T. P. Nugroho, S. Chusniati, and K. Rochiman, “Eksplorasi bakteri selulolitik asal cairan rumen sapi potong sebagai bahan inokulum limbah pertanian,” Jurnal Ilmiah Kedokteran Hewan, vol. 4, no. 1, 2011.
M. Alexander, Introduction to Soil Microbiology. Second Edition. New York, John and Sons, 1976.
R. Khurana, V. N. Uversky, L. Nielsen, and A. L. Fink. (2011). Is Congo red an amyloid specific dye?. Journal of Biological Chemistry. [Online]. Available: http://www.jbc.org/content/276/25/22715.full
R. F. Fadilah, “Isolasi and identifikasi bakteri selulolitik pengurai sampah organik dari berbagai tempat,” Skripsi, Fakultas Ilmu Pengetahuan Alam dan Matematika, Universitas Pendidikan Indonesia, 2012.
Sutoyo and K. Muzakhar, “Skrining dan identifikasi bakteri endosimbiotik dalam usus rayap penghasil selulase,” Jurnal Pengendalian Hayati, vol. 1, no. 2, pp.104-110, 2008.
V.V. Zverlova, W. Holl, and H. Schwarz, “Enzymes for digestion of cellulose and other polysaccharides in the gut of longhorn beetle larvae, Rhagium inquisitor L. (Col., Ccerambycidae).” International Biodeterioration & Biodegredation, vol. 5, no. 3, pp.175-179, 2003.
Fikrinda, “Isolasi dan karakterisasi bakteri penghasil selulase ekstermofilik dari ekosistem air hitam,” Tesis Program Pascasarjana, IPB, Bogor, 2000.
C. L. Aguiar, “Biodegradation of the cellulose from sugarcane bagasse by fungal cellulase,” Journal of Science Technology Aliment, vol. 3, pp. 117-121, 2001.
A. T. Martinez et al., “Biodegradation of lignocellulosics: microbial, chemical and enzymatic aspects of the fungal attack of lignin”. Journal of International Microbiol, vol. 8, no. 3, pp.195-204, 2005.
T. D. Bugg, M. Ahmad., E. M. Hardiman, and R. Singh, “The emerging role for bacterial in lignin degradation and bio-product formation,” Journal of Current Opinion in Biotechnology, vol. 22, pp.1-7, 2010.
A. D. Bholay et al., “Bacterial lignin peroxidase: A tool for biobleaching and biodegradation of industrial effluents,” Journal Environment Res Technol, vol. 2, no.1, pp.58-64, 2012.
R. S. Hadioetomo, Mikrobiologi Dasar dalam Praktek Teknik dan Prosedur Dasar Laboratorium. Jakarta, Gramedia, 1993.
A. Anuar, A. Dahliaty, and C. Jose, “Isolasi bakteri selulolitik dari perairan dumai,” Jurnal Online Mahasiswa, vol. 1, no. 2, 2014.
Rostinawati, “Skrining dan identifikasi bakteri penghasil enzim kitinase dari air laut di perairan pantai Pondok Bali,” Penelitian Mandiri, Fakultas Farmasi, Universitas Padjadjaran, Jatinangor, 2008.
W. A. Volk, Mikrobiologi Dasar. Terjemahan Soenartono Adisoemarto. Jakarta, Erlangga, 1998.
L. Waluyo, Mikrobiologi Umum. Malang, UMM Press, 2004.
A. A. P. Anand et al., “Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and strach and their impact on digestion,” Journal Insect Science, vol. 10, pp.1-20, 2010.
S. Huang, P. Sheng, and H. Zhang, “Isolation and identification of cellulolytic bacteria from the gut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae),” International Journal Molecular Science, vol. 13, pp.2563-2577, 2012.
J. Zhou et al., “Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T100-14,” Journal Bioresour Technology, vol. 99, pp.6826-6833, 2008.
S. M. Geib, M. Tien, and K. Hoover, “Identification of proteins involved in lignocellulose degradation using in gel zymogram analysis combined with Mass Spectroscopy-based peptide analysis of gut proteins from larva Asian plonghorned beetles, Anoplophora glabripennis,” Journal Insect Science, vol. 17, pp.253-264, 2010.
S. L. A. Sari, A. Pangastuti, and S. Winarseh. Penampisan bakteri cellulolytic dari saluran pencernaan Attacus atlas L,” in Proceeding of prosiding Seminar Nasional Mikrobiologi: Keanekaragaman dan Pemanfaatan Sumberdaya Mikroba Tropika Indonesia, 2014, pp.36-40.
