Enhancing Genetic Gain in Potato Clones through Phenotyping Late Blight Resistance
DOI:
https://doi.org/10.32734/injar.v4i2.5997Keywords:
genetic gain, late blight, phenotyping, potato, resistanceAbstract
Late blight, caused by Phytophthora infestans (Mont.) de Bary, is a devastating disease of potato (Solanum tuberosum L.). To identify potential sources of resistance to the disease, 32 clones received from the National Potato Research Program (NPRP) were evaluated under natural conditions at the National Maize Research Program Rampur, Chitwan in 2018 and 2019. Potato cultivars Desire, Kufri Jyoti, and Farmers local were used as moderately resistant, susceptible, and highly susceptible checks, respectively. The experiments were laid out in α-lattice design with two replications. Each experimental plot of 3.6 m2 was seeded as two 3m long rows with 0.6 and 0.25 m row and plant spacing, respectively. Agronomic practices were followed as recommended by NPRP. The disease severity was measured based on a percentage of leaf area infected using disease scale of (1 to 9) at three times in seven days intervals. Disease severity values were converted into the area under disease progress curve (AUDPC). During harvest, the total yield and its components were recorded. Potato clones differed significantly (P ≤ 0.01) for disease severity, yield, and yield components. The results revealed high genetic variability, heritability, and genetic gain for disease parameters, tuber yield, and its components. Six clones (CIP311622.9, PRP277072.122, PRP146971.135, PRP147072.27, CIP311350.27, and PRP146971.117 had lower area under disease progress curve AUDPC) values (274.25 to 421.03), showed higher resistant in both years and yielded more tuber yield (~20 t/ha) than other clones. These clones could be used to develop late blight resistant, high yielding potato varieties
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References
A. Haverkort, F. J. de Ruijter, F. K. van Evert, J. G. Conijn, and B. Rutgers, “Worldwide sustainability hotspots in potato cultivation-Identification and mapping,” Potato Research, vol. 56, pp. 343–353, 2014.
FAOSTAT, “Food balance sheet,” Food and Agriculture Organization, 2013. [Online]. Available: http://www.fao.org/faostat/en/#data/FBS.
MOAD, “Statistical information on Nepalese agriculture 2018/19,” Ministry of Agriculture Development, Kathmandu, Nepal, 2019.
S. Kaur and K. G. Mukerji, “Potato Diseases and their Management,” in Fruit and Vegetable Diseases, vol. 1, K.G. Mukerji, Ed., Kluwer Academic Publishers, Springer, Dordrecht, Netherland, ISBN: 978-1-4020-1976-0, 2004, pp.233-280.
G. Forbes, W. Pérez, and J. A. Piedra, “Field assessment of resistance in potato to Phytophthora infestans,” International Potato Center (CIP), Lima, Peru, 2014.
M. Nowicki, M. R. Foolad, M. Nowakowska, and E. U. Kozik, “Potato and tomato late blight caused by Phytophthora infestans: an overview of pathology and resistance breeding,” Plant Disease, vol. 96, pp. 4–17, 2012.
A. M. Ah-Fong, K. S. Kim, and H. S. Judelson, “RNA-seq of life stages of the oomycete Phytophthora infestans reveals dynamic changes in metabolic, signal transduction, and pathogenesis genes and a major role for calcium signaling in development,” BMC Genomics vol. 18, no. 1, pp. 198-199, 2017.
S.Y. Rodenburg, M. F. Seidl, D. de Ridder and F. Govers, “Genome-wide characterization of Phytophthora infestans metabolism: a systems biology approach,” Molecular Plant Pathology, vol.19, no. 6, pp.1403–1413, 2018.
A. J. Haverkort, P. C. Struik, R. G. F. Visser and E. Jacobsen,“Applied biotechnology to combat late blight in potato caused by Phytophthora infestans,” Potato Research, vol. 52, no.3, pp. 249–264, 2009.
A. H. Sparks, G. A. Forbes, R. J. Hijmans and K. A. Garrett, 2014. “Climate change may have limited effect on global risk of potato late blight,” Global Change Biology, vol. 20, pp. 3621–3631, 2014, doi:10.1111/gcb.12587.
W. W. Kirk, F. M. Abu-El Samen, J. B. Muhinyuza, R. Hammerschmidt, D. S. Douches, C. A. Thill, H. Groza and A. L. Thompson, “Evaluation of potato late blight management utilizing host plant resistance and reduced rates and frequencies of fungicide applications,” Crop Protection, vol. 24, no. 11, pp. 961–970, 2005.
R. Nærstad, A. Hermansen and T. Bjor, “Exploiting host resistance to reduce the use of fungicides to control potato late blight,” Plant Pathology, vol. 56, no. 1, pp. 156–166, 2007.
L. Cooke, H. Schepers, A. Hermansen, R. Bain, N. Bradshaw, F. Ritchie, D. Shaw, A. Evenhuis, G. J. T. Kessel, J. G. N. Wander, B. Andersson, J. G. Hansen, A. Hannukkala, R. Nærstad and B. J. Nielsen, “Epidemiology and integrated control of potato late blight in Europe,” Potato Research, vol. 54, no. 2, pp.183–222, 2011.
E. Liljeroth, A. Lankinen, L. Wiik, D. D. Burra, E. Alexandersson and E. Andreasson, “Potassium phosphite combined with reduced doses of fungicides provides efficient protection against potato late blight in large-scale field trials,” Crop Protection, vol. 86, pp. 42–55, 2016.
A. J. Haverkort, P. M. Boonekamp, R. Hutten, E. Jacobsen, L. A. P. Lotz, G. J. T. Kessel, J. H. Vossen and R. G. F. Visser, “Durable late blight resistance in potato through dynamic varieties obtained by cisgenesis: scientific and societal advances in the DuRPh project,” Potato Research, vol. 59, no. 1, pp. 35–66, 2016.
G. A. Forbes, “Using host resistance to manage potato late blight with particular reference to developing countries,” Potato Research, vol. 55, no. 3–4, pp. 205–216, 2012.
J.W. Henfling, “Late blight of potato: Phytophthora infestans,” Technical Information Bulletin, vol. 4 (2nd ed revised), CIP, Lima, Peru, pp. 22-23, 1987.
G. Shanner and R. E. Finney, ‘The effect of nitrogen fertilization on the expression of slow mildewing resistance in knox wheat,” Phytopathology, vol. 67, pp.1057-1066, 1977.
W. Perez and G. Forbes, Potato late blight technical manual. CIP Publication, Lima, Peru, 2010.
G. A. Burton and E. H. Devane, “Estimation of heritability in tall festuca (Festuca arundinacea) from replicated clonal materials,” Agronomy Journal, vol. 45, pp. 478-479, 1953.
R.W. Allard, Principles of Plant Breeding. John Willy and Sons, Inc., New York, USA, 1960.
D. S. Falconer and T. F. C. Mackay, Introduction to Quantitative Genetics. 4th ed, Benjamin Cummings, Longman Inc., New York, ISBN: 10: 0582243025, 1996.
H. W. Johnson, H. F. Robinson and R. E. Comstock, “Estimates of genetic and environmental variability in soybeans,” Agronomy Journal, vol. 47, pp. 314-318, 1955.
P. A. Miller, J. C. Williams, H. F. Robinson and R. F. Comstock, “Estimation of genotypic and environmental variances and covariances in upland cotton and their implications in selection,” Agronomy Journal, vol. 50, pp. 126-131, 1958.
G. Alvarado, M. Lopez, M. Vargas, A. Pacheco, F. Rodríguez, J. Burgueño and J. Crossa. 2015. META-R (Multi Environment Trial Analysis with R for Windows) Version 5.0 (2015), International Maize and Wheat Improvement Center (Distributor) V13 Version. [0nline] Available: http://hdl.handle.net/11529/10201">hdl:11529/10201.
B.S. Nandipuri, B. S. Singh and T. Lal, “Studies on the genetic variability and correlation of some economic characters in tomato,” Journal of Research, vol. 10, pp. 316-321, 1973.
S. Subedi, “A review on important maize diseases and their management in Nepal,” Journal of Maize Research and Development, vol.1, no.1, pp.28-52, 2015, doi:10.3126/jmrd. v1i1.14242.
S. K. Kaushik, V. Bhardwaj, P. H. Singh and B. P. Singh, “Evaluation of potato germplasm for adaptability and resistance to late blight,” Potato Journal, vol. 34, pp. 43-44, 2007.
R. Sharma, V. Bhardwaj, D. Dalamu, S. K. Kaushik, B. P. Singh, S. Sharma, R. Umamaheshwari, R. Baswaraj, V. Kumar and C. Gebhardt, “Identification of elite potato accessions possessing multiple disease resistance genes through molecular approaches,” Scientia Horticulturae, vol. 179, pp. 204-211, 2014.
NPRP, “Annual report 2072/73 (2015/16),” National Potato Research Program, NARC, Khumaltar, Lalitpur, Nepal, 2016.
A. K. Srivastava, V. Kumar, T. A. Joseph, S. Sharma, T. K. Bag and B. P. Singh, “Screening potato germplasm for stable resistance against late blight (Phytophthora infestans),” Potato Journal, vol. 39, pp. 177-184, 2012.
L. J. Turkensteen, “Durable resistance of potatoes against Phytophthora infestans,” in Durability of disease resistance, T. Jacobs and J. E. Parlevliet, Eds. Kluwer Academic Publishers, Dordrecht, Netherlands, 1993, pp. 115-124.
K. G. Haynes, D. H. Lambert, B. J. Christ, D. P. Weingartner, D. S. Douches, J. E. Backlund, G. Secor, W. E. Fry and W. Stevenson, “Phenotypic stability of resistance to late blight in potato clones evaluated at eight sites in the United States,” American Journal of Potato Research, vol. 75, pp. 211-217, 1998.
V. Umaerus and M. Umaerus, “Inheritance of resistance to late blight,” in Potato Genetics, J. E. Bradshaw and G. R. Mackay, Eds. CAB International, Wallingford, UK, 1994, pp. 365-402.
NPRP, “Annual report 2074/75 (2017/18),” National Potato Research Program, NARC, Khumaltar, Lalitpur, Nepal, 2018.
M. S. Gurjar, S. Sharma and V. Kumar, “Phenotyping of potato accessions for stable resistance to late blight (Phytophthora infestans),” Indian Phytopathology, vol. 69, no. 2, pp. 141-144, 2016.
D. Pattanayak, S. K. Chakrabarti and P. S. Naik, “Genetic diversity of late blight resistant and susceptible Indian potato cultivars revealed by RAPD markers,” Euphytica, vol. 128, pp. 183–189, 2002.
E. M. Abou-Taleb, M. Sayed, M. E. Aboshosha and H. E. Mahmoud, “Genetic diversity among late blight resistant and susceptible potato genotypes,” Saudi Journal of Biological Science, vol. 17, no. 2, pp. 133–138, 2010.
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