ANTIOXIDANT ACTIVITY OF HYDROALCOHOLIC EXTRACT OF GREEN GEDI LEAVES (Abelmoschus manihot L.) USING CUPRAC (CUPRIC ION REDUCING ANTIOXIDANT CAPACITY) METHOD
DOI:
https://doi.org/10.32734/idjpcr.v8i02.20644Keywords:
Abelmoschus manihot L., antioxidant activity, CUPRAC, hydroalcoholic extract, IC50Abstract
Free radicals induce oxidative stress, which plays a pivotal role in the pathogenesis of various degenerative diseases. Abelmoschus manihot L., locally known as green gedi, is recognized for its rich secondary metabolite content with potential antioxidant properties. This study aimed to evaluate the antioxidant activity of the hydroalcoholic extract of green gedi leaves using the Cupric Ion Reducing Antioxidant Capacity (CUPRAC) method. Extraction was conducted via maceration using a 90% hydroalcoholic solvent, followed by comprehensive phytochemical screening and simplicia characterization. The antioxidant capacity was quantified by determining the IC50 value through linear regression analysis of inhibition percentage versus concentration. Phytochemical analysis confirmed the presence of alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids/steroids. The hydroalcoholic extract exhibited "very strong" antioxidant activity, yielding an IC50 value of 41.65 ± 0.30 µg/mL. Although this potency was slightly lower than the pure quercetin standard, it demonstrates a significant capacity for free radical neutralization. These findings underscore the potential of Abelmoschus manihot L. as a robust natural antioxidant source, supporting its further development into phytopharmaceuticals or functional health supplements for the prevention of oxidative stress-related conditions.
Downloads
References
[1] A. Dalimunthe, P. A. Z. Hasibuan, J. Silalahi, S. F. Sinaga, and D. Satria, ‘Antioxidant activity of alkaloid compounds from Litsea cubeba Lour’, Oriental Journal of Chemistry, vol. 34, no. 2, p. 1149, 2018.
[2] N. Nasri, V. E. Kaban, H. D. Syahputra, J. G. Ginting, and C. G. Tania, ‘Peningkatan Pengetahuan Pola Hidup Bersih dan Sehat serta Penggunaan Vitamin pada Anak di Panti Asuhan Claresta’, ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi, vol. 2, no. 1, pp. 145–153, 2023.
[3] M. Y. Alkandahri, L. Nisriadi, and E. Salim, ‘Secondary metabolites and antioxidant activity of methanol extract of Castanopsis costata leaves’, Pharmacology and Clinical Pharmacy Research, vol. 1, no. 3, pp. 98–102, 2016.
[4] V. E. Kaban and S. Yusmarlisa, ‘Uji Aktivitas Kandungan Antioksidan Pada Daun Bangun-Bangun (Plectranthus amboinicus) Secara Spektrofotometri Ultraviolet-Visible’, Jurnal farmasimed (JFM), vol. 1, no. 1, pp. 16–20, 2018.
[5] U. Harahap, A. Dalimunthe, T. Hertiani, M. Muhammad, N. Nasri, and D. Satria, ‘Antioxidant and antibacterial activities of ethanol extract of Vernonia amygdalina Delile. Leaves’, in AIP Conference Proceedings, AIP Publishing, 2021.
[6] M. F. Lubis, H. Syahputra, D. N. Illian, and V. E. Kaban, ‘Antioxidant activity and nephroprotective effect of Lansium parasiticum leaves in doxorubicin-induced rats’, J Res Pharm, vol. 26, no. 3, pp. 565–573, 2022.
[7] D. HIDAYANTI, ‘Skrining Fitokimia, Kadar Zat Gizi Dan Daya Antioksidan Ekstrak Daun Gedi Hijau (Abelmoschus Manihot L)’, Universitas Tadulako, 2022.
[8] M. N. Warongan, ‘Analisis Fingerprint Daun Gedi Hijau (Abelmoschus Manihot L) Untuk Memprediksi Aktivitas Antioksidan Menggunakan Kombinasi Spektroskopi Ir Dengan Partial Least Squareregression’, Pharmacon, vol. 6, no. 4, 2017.
[9] V. E. Kaban, N. A. Thomas, and I. Sholihah, ‘PENGANTAR ILMU FARMASI’, Accessed: Aug. 08, 2024. [Online]. Available: https://www.researchgate.net/profile/Andi-Asari/publication/372717263_PENGANTAR_ILMU_FARMASI/links/64c3c3b0141074110edf02cc/PENGANTAR-ILMU-FARMASI.pdf
[10] N. Nasri, V. E. Kaban, D. Satria, H. D. Syahputra, and Z. Rani, ‘Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi’, Journal of Pharmaceutical and Health Research, vol. 4, no. 1, pp. 79–84, 2023.
[11] M. Safithri, D. Nur Faridah, F. Ramadani, and R. Pratama, ‘Antioxidant activity of ethanol extract and fractions of Piper crocatum with Rancimat and cuprac methods’, Turkish Journal of Biochemistry, vol. 47, no. 6, pp. 795–801, 2022.
[12] C. Irawan, I. Dwi Putri, and M. Sukiman, ‘Antioxidant activity of DPPH, CUPRAC, and FRAP methods, as well as activity of alpha-glucosidase inhibiting enzymes from Tinospora crispa (L.) stem ultrasonic extract’, 2022.
[13] R. Nobertson, N. P. Indah, and Y. S. Kenta, ‘Uji Aktivitas Antioksidan Ekstrak Etanol Daun Gedi Merah (Abelmoschus Manihot (L).) Palu Sulawesi Tengah’, Farmakologika: Jurnal Farmasi, vol. 15, no. 1, pp. 63–71, 2018.
[14] D. Satria, J. Silalahi, G. Haro, S. Ilyas, and P. A. Z. Hsb, ‘Antioxidant and antiproliferative activities of an ethylacetate fraction of Picria fel-terrae Lour. herbs’, Asian Pacific journal of cancer prevention: APJCP, vol. 18, no. 2, p. 399, 2017.
[15] G. M. El-Sherbiny, A. M. Gazelly, M. H. Sharaf, S. A. Moghannemm, M. K. Ismail, and A. S. El-Hawary, ‘Exploitation of the antibacterial, antibiofilm and antioxidant activities of Salvadora Persica (Miswak) extract’, Journal of Bioresources and Bioproducts, vol. 8, no. 1, pp. 59–65, 2023.
[16] L. Marinelli et al., ‘In vitro wound-healing properties of water-soluble terpenoids loaded on halloysite clay’, Pharmaceutics, vol. 13, no. 8, p. 1117, 2021.
[17] M. E. T. Butar-Butar, H. D. Syahputra, N. R. K. Nisaa, V. E. Kaban, and H. L. Priatni, KIMIA FARMASI. Get Press Indonesia, 2023.
[18] E. S. Rumaseuw et al., Farmakognosi. Global Eksekutif Teknologi, 2023.
[19] F. E. Hasibuan, W. Syahfitri, S. Ilyas, and S. Hutahaean, ‘Phytochemical screening, antioxidant activity and thin-layer chromatography test of methanol extract and simplicia leaves of loquat (Eriobotrya japonica Lindl)’, in IOP Conference Series: Materials Science and Engineering, IOP Publishing, 2020, p. 012069. Accessed: Oct. 17, 2024. [Online]. Available: https://iopscience.iop.org/article/10.1088/1757-899X/725/1/012069/meta
[20] U. K. Karmakar, J. A. Shilpi, M. H. Hossain, and P. Kundu, ‘PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF PORTULACA OLERACEA LINN.’, Khulna University Studies, pp. 30–44, 2021.
[21] L. S. Idrus et al., ‘Potensi Hepatoprotektor dan Antioksidan dari Rimpang Olae (Etlingera calophrys (K. Schum) AD Poulsen)’, Jurnal Mandala Pharmacon Indonesia, vol. 9, no. 2, pp. 475–483, 2023.
[22] R. F. R. Situmorang, K. Gurning, V. E. Kaban, M. J. Butar-Butar, and S. A. B. Perangin-Angin, ‘Determination of total phenolic content, analysis of bioactive compound components, and antioxidant activity of ethyl acetate Seri (Muntingia calabura L.) leaves from North Sumatera Province, Indonesia’, Open Access Macedonian Journal of Medical Sciences, vol. 10, no. A, pp. 240–244, 2022.
[23] E. D. L. Putra, H. Cintya, and D. Satria, ‘Antibacterial and antioxidant activities of ethanol extract of sukun (Artocarpus altilis.) leaves against Pseudomonas aeruginosa’, in E3S Web of Conferences, EDP Sciences, 2021, p. 08006.
[24] W. Marampung, H. E. I. Simbala, and E. M. Rumondor, ‘Analisis GC-MS (Gas Chromatography-Mass Spectrometry) dari Ekstrak Etanol Daun Gedi Hijau (Abelmoschus manihot L.)’, Jurnal Lentera Farma, vol. 3, no. 1, pp. 07–13, 2024.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Indonesian Journal of Pharmaceutical and Clinical Research
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR) and Faculty of Pharmacy as well as TALENTA Publisher Universitas Sumatera Utara as publisher of the journal.
Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, will be allowed only with a written permission from Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR).
The Copyright Transfer Form can be downloaded here.
The copyright form should be signed originally and sent to the Editorial Office in the form of original mail or scanned document.
