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A Detection of Gemini Virus on Tomato Plants (Solanum lycopersicum Mill.) in Karo North Sumatera Using Molecular Techniques

Authors

  • Saleha Hannum
  • Suci Rahayu Dapartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Jl. Bioteknologi No. 1 Medan 20155, Indonesia.
  • Nurainun Dapartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Jl. Bioteknologi No. 1 Medan 20155, Indonesia.
  • Sofi Junita Rispauli Damanik Dapartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Jl. Bioteknologi No. 1 Medan 20155, Indonesia.

DOI:

https://doi.org/10.32734/ijoep.v6i2.17932

Keywords:

Karo North Sumatera, Solanum lycopersicum Mill., Gemini virus, Polymerase Chain Reaction

Abstract

 Tomatoes are susceptible plants to various plant pests. One of the obstacles in tomato cultivation in Karo North Sumatera is the interference from various microorganisms such as plant virus attacks. The symptoms are the same as other diseases when in the field which causes difficulties in determining the type of virus that causes a disease, so it is necessary to carry out further testing as a good prevention. This study used the Polymorphism Chain Reaction (PCR) technique with universal primers for the Gemini virus. All samples of diseased tomato leaves originating from the Karo were attacked by Geminivirus which correlated with the presence of a DNA band measuring 1.5 kb after amplified.

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Published

2024-09-27

Versions

How to Cite

Hannum, S., Rahayu, S., Nurainun, & Damanik, S. J. R. (2024). A Detection of Gemini Virus on Tomato Plants (Solanum lycopersicum Mill.) in Karo North Sumatera Using Molecular Techniques. International Journal of Ecophysiology, 6(2), 74-81. https://doi.org/10.32734/ijoep.v6i2.17932