The Number of Bacteria Colonies in Carp Fish (Cyprinus carpio) After Administration of Lime (Citrus aurantifolia) and Orange Extract (Citrus jambhiri Lush.)


  • Nenni Dwi Aprianti Lubis Universitas Sumatera Utara



Carp, extract, lime, rough lemon, total colonies


Fish is one of the most commonly consumed protein sources. Fish are at risk as a medium for the growth of microbial contaminants that can cause foodborne diseases. This disease can cause diarrhea and even death.Contaminant microbes can be found in foods consumed raw, such as Naniura, a typical Batak cuisine in the form of raw carp. Various natural spices such as lime and orange also have potential as antimicrobials. This study aimed to determine the antimicrobial potential of lime and orange juice in fresh carp samples. This study used extracts of lime and orange with concentrations of 6.25%, 12.5%, 25%, and 50%, respectively. The sample used is carp meat. The bacteria present in the sample were counted using the Total Plate Count (TPC) method before and after lime and lime extract administration. The results showed that before administering the two extracts, the total colonies in the sample could not be counted. The antimicrobial effect of orange juice extract began to be seen at a concentration of 12.5%, while in lime, it was only seen at a concentration of 50%. From the results of this study, it was concluded that orange juice extract had a better antimicrobial effect than lime.


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Kementerian Kelautan dan Perikanan Republik Indonesia. 2020 KKP Targetkan Konsumsi Ikan 56,39 kg. [online] Available from:

Amelia S, Lubis NDA, Balatif R. Mikroorganisme dan Bahan Pangan. Medan: Penerbit Qiara Media; 2020

Novoslavskij A, Terentjeva M, Eizenberga I, Valcina O, Bartkevics V, Berzins A. Major foodborne pathogens in fish and fish products: a review. Annals of Microbiology.; 66, pp. 1-15. Doi: 10.1007/s13213-015-1102-5.2016

Amelia S, Lubis NDA, Balatif R, Rozi MF, Sidhi SP. Antibacterial effect of Andaliman (Zanthoxylum acanthopodium) against contaminant in raw common carp (Cyprinus carpio Linnaeus). IOP Conf. Series: Earth and Environmental Science.; Vol. 425, 012036. Doi: 10.1088/1755-1315/425/1/012036. 2020

Hutapea CN, Leksono T, Sari NI. The effect of different kind of citrus on the quality of Jelawat fish (Leptobarbus hoevenii) Naniura. Berkala Perikanan Terubuk. Vol. 47, No. 2, pp.165-175. 2019.

Sembiring HB, Sihotang H, Tampubolon AC. Antibacterial Activities of Rough Lemon (Citrus jambhiri Lush) Rind Essential Oil. Journal of Chemical Natural Resources. Vol. , No. 1, pp. 12-18 .2019

Lemes RS, Alves CCF, Estevam EBB, Santiago MB, Martins CHG, Santos TCLC, et al. Chemical composition and antibacterial activity of essential oils from Citrus aurantifolia leaves and fruit peel against oral pathogenic bacteria. An Acad Bras Cienc.;Vol. 90. No. 2, pp. 1285-1292. Doi: 10.1590/0001-3765201820170847. 2018

Rasul, M.G., Conventional extraction methods use in medicinal plants, their advantages and disadvantages. Int. J. Basic Sci. Appl. Comput, Vol. 2, pp.10-14. 2018.

Jongenburger, Reij, Boer, Gorris, dan Zwietering, Factors Influencing The Accuracy of The Plating Method Used to Enumerate Low Numbers of Viable Microorganism in Food. International. Journal of Food Microbiology. Vol. 143, pp. 32-40. 2010.

Barrett KA, Nakao JH, Taylor EV, Eggers C, Gould LH. Fish-Associated Foodborne Disease Outbreaks: United States, 1998-2015. Foodborne Pathog Dis. Sep;Vol.14. No. 9. pp. 537-543. doi: 10.1089/fpd.2017.2286. 2017

Yap PS, Yusoff K, Lim SH, Chong CM, Lai KS. Membrane Disruption Properties of Essential Oils—A Double-Edged Sword?. Processes. Vol. 9, No. 4, pp. 595. doi:10.3390/pr9040595. 2021.

Li ZH, Cai M, Liu YS, Sun PL, Luo SL. Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules. Vol. 24, No. 8, pp. 1577. doi:10.3390/molecules24081577. 2019



How to Cite

Lubis, N. D. A. (2022). The Number of Bacteria Colonies in Carp Fish (Cyprinus carpio) After Administration of Lime (Citrus aurantifolia) and Orange Extract (Citrus jambhiri Lush.). Journal of Saintech Transfer, 5(1), 29-33.