Analyses of Bioactive Compounds of Pegagan (Centella Asiatica (L.) Urb) from Samosir – Indonesia Accession
Keywords:asiaticoside, asiatic acid, madeccasoside
Centella Asiatica or Pegagan is classified as one of the wild plants that has not been domesticated. The excessive usage of this plant in traditional and modern medicinal applications threatens its population and sustainability. Thus, to preserve the plant and supply the high request of this plant in agromedicinal industry, studies concerned with the growth and bioactive compounds of Pegagan cultivated under commercial field conditions are urgently needed. This study purposed to examine the bioactive components of Pegagan (especially in leaves and roots) under field conditions, including asiaticoside, madeccasoside, and Asiatic acid. The Pegagan was harvested weeks after planting (WAP). The wet and dry weights of the leaves and roots were weighted and subsequently measured for their centelloside compounds by Thin-Layer Chromatography (TLC) procedure. The results revealed that the resulting asiaticoside content in the roots (1.25%) was higher than in the leaves (0.88%). The same results were achieved for the madecassoside content where the madecasosside content in the roots was 2.23%, while the content in the leaves was 2.11%. However, contrarily, the Asiatic acid compound in the leaves was 1.10% higher than the content in the roots (0.60%). It might be attributed to a longer period of field cultivation of Pegagan that delivered adequate time for the plant to alter Asiatic acid to asiaticoside and madecasosside at a later developmental growth. Moreover, these discoveries are advantageous in defining the most proper harvest time for commercial field cultivation of Pegagan to yield the highest amount of certain centelloside compounds.
G. Alam and J. Belt, “Developing a medicinal plant value chain: Lessons from an initiative to cultivate Kutki (Picrorhiza kurrooa) in Northern India,” The Journal of Infection in Developing Countries, 2009.
A. Ziemienowicz, “Agrobacterium-mediated plant transformation: Factors, applications and recent advances,” Biocatalysis and Agricultural Biotechnology, vol. 3, no. 4, 2014.
J. S. Duhan, R. Kumar, N. Kumar, P. Kaur, K. Nehra, and S. Duhan, “Nanotechnology: The new perspective in precision agriculture,” Biotechnology Reports, vol. 15, pp. 11-23, 2017.
M. V. Sudhakaran, “Botanical pharmacognosy of Centella Asiatica (Linn.) Urban,” Pharmacogn. J., vol. 9, no. 4, 2017.
S. Jisha, K. N. Anith, and K. K. Sabu, “The protective role of Piriformospora indica colonization in Centella asiatica (L.) in vitro under phosphate stress,” Biocatal. Agric. Biotechnol., vol. 19, 2019.
M. Rafi et al., “A combination of simultaneous quantification of four triterpenes and fingerprint analysis using TLC for rapid identification of Centella asiatica from its related plants and classification based on cultivation ages,” Ind. Crops Prod., vol. 122, pp. 93-97, 2018.
A. Alqahtani, W. Tongkao-On, K. M. Li, V. Razmovski-Naumovski, K. Chan, and G. Q. Li, “Seasonal Variation of Triterpenes and Phenolic Compounds in Australian Centella asiatica (L.) Urb,” Phytochem. Anal., vol. 26, no. 6, pp. 436-443, 2015.
T. Nazir, A. K. Uniyal, and N. P. Todaria, “Allelopathic behaviour of three medicinal plant species on traditional agriculture crops of Garhwal Himalaya, India,” Agrofor. Syst., vol. 69, pp. 183–187, 2007.
P. Nautiyal, R. Rajput, D. Pandey, K. Arunachalam, and A. Arunachalam, “Role of glomalin in soil carbon storage and its variation across land uses in temperate Himalayan regime,” Biocatal. Agric. Biotechnol., vol. 21, 2019.
Li-Na Zhang et al., “Protective effects of asiaticoside on septic lung injury in mice,” Exp. Toxicol. Pathol., vol. 63, no. 6, pp. 519-25, 2011.
I. E. Orhan, “Centella asiatica (L.) Urban: From traditional medicine to modern medicine with neuroprotective potential,” Evidence-based Complementary and Alternative Medicine. 2012.
G. Suresh et al., “Mycosynthesis of anticancer drug taxol by Aspergillus oryzae, an endophyte of Tarenna asiatica, characterization, and its activity against a human lung cancer cell line,” Biocatal. Agric. Biotechnol., vol. 24, 2020.
S. Mangas, E. Moyano, L. Osuna, R. M. Cusido, M. Bonfill, and J. Palazón, “Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica,” Biotechnol. Lett., vol. 30, no. 10, pp. 1853-1859, 2008.
J. T. James and I. A. Dubery, “Pentacyclic triterpenoids from the medicinal herb, Centella asiatica (L.) Urban,” Molecules, vol. 14, no. 10, pp. 3922-3941, 2009.
J. Satheesan S. Jisha, K. N. Anith, and Sabu K. K., “Corrigendum to the protective role of Piriformospora indica colonization in Centella asiatica (L.) in vitro under phosphate stress,” Biocatalysis and Agricultural Biotechnology, vol. 31, 2020.
U. S. Bagde, R. Prasad, and A. Varma, “Interaction of Mycobiont: Piriformospora indica with medicinal plants and plants of economic importance,” African Journal of Biotechnology, vol. 9, no. 54, 2010.
A. Hikmat, E. A.M. Zuhud, Siswoyo, E. Sandra, and R. K. Sari, “Revitalisasi konservasi tumbuhan obat keluarga (toga) guna meningkatkan kesehatan dan ekonomi keluarga mandiri di desa contoh lingkar kampus IPB darmaga bogor,” J. Ilmu Pertan. Indones., vol. 16, no.2, 2011.
N. S. Vinolina, M. Nainggolan, and R. Siregar, “Production enhancement technology of Pegagan (Centella asiatica),” Agrivita, vol. 4, no.2, 2018.
M. Srivastava, G. Singh, and P. Misra, “Contribution of biotechnological tools in the enhancement of secondary metabolites in selected medicinal climbers,” in Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers, Shahzad, A., Sharma, S., Siddiqui, S., Ed., Springer, 2015, pp 465–486.
O. T. Kim, M. Y. Kim, M. H. Hong, J. C. Ahn, and B. Hwang, “Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) urban by elicitors.,” Plant Cell Rep., vol. 23, no. 5, pp. 339-44, 2004.
E. Lambert, A. Faizal, and D. Geelen, “Modulation of triterpene saponin production: In vitro cultures, elicitation, and metabolic engineering,” Appl. Biochem. Biotechnol., vol. 164, pp. 220–237, 2011.
S. Mangas et al., “The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants,” Phytochemistry, vol. 67, no. 18, pp. 2041-2049, 2006.
N. S. Vinolina, “Secondary Metabolite Content in Pegagan (Centella asiatica) from North Sumatera,”, J. Phys.: Conf. Ser. 1175 012003, 2019.
KRI Departemen. Materia Medika Indonesia Jilid VI. Depkes RI, Jakarta, 1995.