PENINGKATAN AKTIVITAS FAGOSITOSIS MAKROFAG MENCIT BALB/C DENGAN IMUNOSUPRESI DAN DIINFEKSI C.ALBICANS AKIBAT PEMBERIAN MINYAK BIJI MIMBA

INCREASING MACROPHAGE PHAGOCYTOSIS ACTIVITY IN BALB/C MICE THAT IMMUNOSUPRESSED AND INFECTED WITH C.ALBICANS CAUSED BY OLEUM AZADIRACHTY

Abstract

Majority of fungal infection in mouth is caused by C.albicans. This case increases parallel with increasing the number of
immunosupressed patient. Mimba (Azadirachta indica) is herbal medicine that has been used as antifungal drug. Beside
that, mimba has immunostimulatory effect. Macrophage phagocytosis, it’s one of natural immunities that plays an
important role in the first immune response againts C.albican infection.The purpose of this research was to know the
effect of oleum azadirachty in macrophage phagocytosis activity of BALB/c mice that immunosupressed and infected by
C.albicans. This laboratory experimental research used BALB/c mice that immunosupressed and infected by C.albicans,
divided into three groups: group 1 (negative control), group 2 (positive control, were immunosupressed and infected with
C.albicans), group 3 (was immunosupressed, infected with C.albicans, and given 75 μl oleum azadirachty). Mice were
immunosupressed given by 2-3mg/mice subcutan injection of prednisolon one day before infected. Group 2 and group 3
were given tetracycline hydrochloride in drinking water (0,83mg/ml) since one day before infected. C.albicans
inoculation was given at zero day. On the 2nd day post infection, mice were acclimated and the macrophage phagocytosis
activity was evaluated by latex beat. Data was analysed by One Way Anova test and LSD (p=0.05). The result showed
that there was significant difference between group 2 and group 3. In conclusion, oleum azadirachty could increase
macrophage phagocytosis activity.