Analysis of biochemical and bioactive content of red rice cv Sidikalang through gamma ray irradiation induction (M4)
DOI:
https://doi.org/10.32734/injar.v9i1.21185Keywords:
bioactive, biochemistry, cv Sidikalang, gamma rays, red riceAbstract
Red rice derives its characteristic color from anthocyanin pigments located in the pericarp and outer endosperm layers, which contribute to its biochemical and bioactive properties. Gamma-ray irradiation is widely applied as a mutation induction technique to enhance genetic variability and improve functional traits in crop plants. This study aimed to evaluate the biochemical and bioactive content of the red rice cv Sidikalang following gamma-ray irradiation. The experiment was conducted at the Central Laboratory, Faculty of Agriculture, Universitas Sumatera Utara, from October 2024 to February 2025, using a single-factor Randomized Block Design with irradiation doses of 0, 200, 400, and 600 Gy and three replications. Biochemical and bioactive parameters analyzed included carbohydrate, protein, fat, vitamin A, fiber, ash, water content, and anthocyanin. The results showed that gamma-ray irradiation had no significant effect on protein, fat, vitamin A, and carbohydrate content, but significantly affected water content, fiber, ash, and anthocyanin. Irradiation doses of 200-600 Gy resulted in significantly higher fiber and anthocyanin content compared to the control. These findings indicate that gamma-ray irradiation has the potential to enhance specific functional and nutritional traits of red rice, providing valuable insights for mutation breeding programs aimed at developing nutritionally improved rice varieties.
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[1] Pusat Data dan Sistem Informasi Pertanian Sekretariat Jenderal Pertanian. 2023, “Statistik konsumsi pangan tahun 2023”. [Online]. Available: https://satudata.pertanian.go.id/details/publikasi/479.
[2] H. Afza, “Peran konservasi dan karakterisasi plasma nutfah padi beras merah dalam pemuliaan tanaman”, Jurnal Litbang Pertanian,vol. 35, no. 3, pp. 143-153, 2016.
[3] W. Yokohama, “Nutritional properties of rice bran, in Champagne, E.T. (Ed). Rice chemistry and technology”, American Association of Cereal Chemists. St. Paul, M.N. pp. 595-609. 2004.
[4] C. K. Reddy, L. Kimi, Haripriya, and N. Kang “Effects of polishing on proximate composition, mineral composition, and antioxidant properties of pigmented rice”, Rice Science, vol. 24, pp. 241-252, 2017.
[5] M. W. Zhang, R. F. Zhang, F. X. Zhang, R. H. Liu, “Phenolic profiles and antioxidant activity of black rice brand of different commercially available varieties”, Journal Agric. Food Chemistry, vol. 58, pp. 7580-7587, 2010.
[6] S. D. Indrasari, P. Wibowo, and E. Y. Purwani, “Evaluasi mutu fisik, mutu giling dan kandungan antosianin kultivar beras merah”. Jurnal Penelitian Pertanian Tanaman pangan, vol. 29, pp. 56-62. 2010.
[7] Damanhuri, “Pewarisan antosianin dan tanggap klon tanaman ubi jalar (Ipomoea batatas (L.) terhadap lingkungan tumbuh”, Disertasi, Universitas Brawijaya, Malang, p. 106, 2005.
[8] R. Suliartini, W. Gusti, Teguh, dan Muhidin, “Pengujian kadar antosianin padi gogo beras merah hasil koleksi plasma nutfah Sulawesi Tenggara”, Jurnal Crop Agro, vol. 4, pp. 43-48, 2011.
[9] F. Pangerang, “Kandungan gizi dan aktivitas antioksidan beras merah dan beras hitam padi ladang lokal dari Kabupaten Bulungan, Provinsi Kalimantan Utara”, Journal of Tropical Agri Food, vol. 3, no.2. pp. 93-100, 2021.
[10] B. O. Juliano “Structure, chemistry and function of the rice grain and its fractions”, Cereal Foods World, vol. 37, no. 10. pp. 772-779, 1992.
[11] F. Chen F, C. Yang, L. Liu, T. Liu, and Y. Wang “Differences, correlation of compositions, taste and texture characteristics of rice from Heilongjiang China”, Journal Rice Res, vol. 5, no.1, pp. 1-5, 2017.
[12] [BPOM] Badan Pengawas Obat dan Makanan Republik Indonesia, “Peraturan Kepala BPOM RI No. HK.03.1.23.11.11.096005 Tahun 2011”, Jakarta, Indonesia, 2011.
[13] D. Naibaho, E. Purba, D.S. Hanafiah, and S. Hasibuan, S, “Improvement of morphology, biochemical characters, and molecular changes of local upland rice cv. Sidikalang M3 generation through induction of gamma–ray irradiation”, Biodiversitas, vol. 24 910, pp. 200-207, 2023.
[14] S. I. Aisyah, in Syukur M, Sastrosumarjo S., (Eds) “Sitogenetika tanaman”, IPB Press, Bogor. pp. 197-209, 2013.
[15] J. A. M. Parry et al., “Review paper: Mutation discovery for crop improvement”, Journal of Experimental Botany, vol. 60. no. pp. 2817-2825, 2009.
[16] K. Archanachai, S. Teepoo and S. Sansenya, “Effect of gamma irradiation on growth proline content, bioactive compound change, and biological activity of 5 populer Thai rice cultivars”, Journal of Biosciense and Bioegineering, vol. 132, pp. 372-380, 2021.
[17] S. Sudarmadji, B. Haryono dan Suhardi, “Analisa Bahan Makanan dan Pertanian. Liberty dan Pusat Antar Universitas Gajah Mada, Yogyakarta.
[18] [AOAC] Association of Official Analitycal Chemist, “Official method of analysis of the association of official analitycal of chemist”. Arlington, Virginia, USA: Published by The Association of Analitycal Chemist, Inc.1995.
[19] S. N. Shanita, H. Hasnah, C.W. Khoo, “Amylose and amylopectin in selected Malaysian food and its relationship to glycemic index”, Sains Malaysiana, vol. 40, no.8, pp. 863-870, 2011.
[20] I. Klimzak, M. Malecka, B. Pacholok “Antioxidant activity of ethanolic extracts of amaranth seeds”, Nahrung/Food, vol. 46930, pp. 184-186, 2002.
[21] J. Lee, R. Durs and R.E. Wrolstad. “Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the ph differential method: Collaborative study”, Journal of AOAC International. vol. 88, no. 5, 2005.
[22] N. Sultan, F.A. Masoodi, I.A. Wani, “Moisture mediated effects of γ-irradiation on physicochemical, functional, and antioxidant properties of pigmented brown rice (Oryza sativa L.) flour”, Journal of Cereal Science, vol. 79. 2017.
[23] Winarno, F. G., “Kimia pangan dan gizi”, PT. Gramedia Utama, Jakarta. 2004.
[24] [BSN] Badan Standardisasi Nasional. SNI Tepung Beras 3549-2009. Dewan Standardisasi Nasional, Jakarta, 2009.
[25] R. Tiwi., A.Yunus., P. Parjanto, “Daya hasil dan kandungan protein mutan M6 padi varietas mentik susu hasil iradiasi sinar gamma”. Agrotechnology Research Journal. vol.1, no.1, pp. 34-40, 2023.
[26] P. Wang P, Y. Zhang, L. Zhao, B. Mo, T. Luo, “Effect of gamma rays on Sophora davidii and detection of DNA polymorphism through ISSR marker”, BioMed Res Intern., pp. 1–6, 2017.
[27] M. A. Esnault, F. Legue and C. Chenal, “Ionizing radiation: Advances in plant response. Environmental”, Experimental Botany, vol. 68, no.3. pp. 231-237, 2010.
[28] D. Marcu, G. Damian, C. Cosma and V. Cristea, “Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays)”, Journal Biology and Physics, vol. 39, no. 4, pp. 625-634, 2013.
[29] M. P. Anugrah, “Pengaruh iradiasi sinar gamma terhadap protein kasar, serat kasar dan lemak kasar tanaman Indigofera zollingeriana”, Skripsi. Fakultas Pertanian Universitas Hasanuddin Makassar, 2021.
[30] I. W. Atmarazaqi. Analisis fenotip dan kandungan antosianin tanaman rosella merah (Hibiscus sabdariffa L.) Pasca iradiasi sinar gamma. Skripsi, UIN Sunan Kalijaga, 2013.
[31] S. T. Jan, T. O. Paarween, Siddiqi, and Mahmooduzzafar, “Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products”. Environmental Journal Review, vol. 20, pp. 17-39, 2012.
[32] F. Li, A. Shimizu, T. Nishio, N. Tsutsumi, and H Kat, “Comparison and characterization of mutations induced by gamma-ray and carbon-ion irradiation in rice (Oryza sativa L.) using whole-genome resequencing”, G3 (Bethesda). vol. 5, no. 11, pp. 3743-3751, 2019.
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