EFFECT OF CORAL GONIOPORA IN COMPARISON WITH CORAL APATITE TOWARDS HUMAN DENTAL PULP STEM CELLS MINERALIZATION ACTIVITIES
EFEK CORAL GONIOPORA DIBANDINGKAN DENGAN CORAL APATITE TERHADAP AKTIVITAS MINERALISASI SEL STEM PULPA GIGI
DOI:
https://doi.org/10.32734/dentika.v17i1.1852Keywords:
dental pulp, coral goniopora, coral apatit, mineralizationAbstract
Challenging approach in tissue engineering for dentin regeneration is focused upon the application of a scaffold on an
open pulp enabling odontoblast-like cells to grow into the scaffold and to convert them into dentin-like substance. Coral
was chosen as a scaffold because of its good biocompatibility and resorbability. The species of marine invertebrates
exploited in medical applications are Members of Porites and Goniopora. Coral goniopora is most marine invertebrata
found in Indonesia's marine. Coral apattite, an osteoconductive synthetic bone graft substitute material, is manufactured
by the hydrothermal conversion of the calcium carbonate skeleton of coral to hydroxyapattite in the presence of
ammonium phosphate preserving the original porous structure which is similar to that of bone. The aim of study was to
investigate the effect of Coral goniopora and coral apattite as a potential scaffold on dental pulp mineralization activity. In
vitro DPSCs mineralization activity was measured by von Kossa staining for calcium deposit identification. The result
that Coral apattite increased more calcium deposited identification than coral goniopora. Calcium deposited on dental
pulp stem cells are marker for mineralized dental pulp stem cells (DPSCs). Mineralized DPSCs are marker for
odontoblast diferentiation and maturation. In conclusion, these observations demonstrated that co-cultured coral apattite
and DPSCs induced a better mineralization activity than those cultured with Coral goniopora.