Application of the flower of Beauveria bassiana Through immersion Seeds and It's effect on Colonization and content of Chlorophil leaves of red chilli (Capsicum annuum L.)
DOI:
https://doi.org/10.32734/jpt.v8i2.6519Keywords:
Colonization, Chlorophyl Content, Beauveria bassiana, Red chiliAbstract
Endophytic fungi in lately was getting a lot of attention in agriculture. Besides being able to increase plant resistance against plant pest organism, it has also the ability to stimulate germination and pant growth. One of endophytic fungi tested in its research was Beauveria bassiana from isolate Wheat, Coffeae, Cacao and entomopatogen fungus from Leptocorisa acuta insect. The aim of this research was to know the ability of Beauveria bassiana from some tested isolates and its effect on chlorophyll content of leaf chili followed Mack Kinner methode by doing seed treatment aplication. Colonization and chlorophyl content of chili leaf was done on 7 MPI. The research design used Random Block Design non factorial, data was analysis by 8 STAT. The result showed all of Beauveria bassiana isolates were able to colonize and established as endophytic in chili plant.Beauveria bassiana of wheat isolate was the best isolate giving spurring germination and seedling plant growth and not significant with B.bassiana isolate from Leptocorisa acuta insect. Aplication of B.bassiana isolates was able to increase the chlorophyll contentof chilileaf compared control. All of the tested B.bassiana fungus could increased chlorophyl-b content and chlorophyl totally of chili leaf, whereas chlorophyl-a content all of tested isolates was not significant. From its research showed there were corelation between colonization and leaf chlorophyll content, the higher percentage of endophytic fungus on leaf chili, it made higher the chlorophyl-b content and chlorophyl totally content of leaf chili.
Downloads
References
Agustiansyah, Ilyas, S, Sudarsono & Machmud, M. (2013).Perlakuan Benih dengan Agen Hayati dan Pemupukan untuk Meningkatkan Pertumbuhan Tanaman, Hasil, dan Mutu Benih Padi, J. Agron.Indonesia, vol.41, no.2.hlm.104-98.
Anand, R, Paul, I& Chanway, C. (2006). Research on endophytic bacteria: Recent advances with forest trees. Soil Biology: Mikrobial root Endophytes, vol.9,pp.106-89
Azarmi, R., B. Hajieghrari, and A. Giglou. (2011). Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. African Journal of Biotechnology. 10(31):5850-5855.
Bacon, C.W., I.E, Yates, D.M.Hinton,F. Meredith.( 2001). Biological Control of Fusarium moniliforme in mayze. Environ Health Perspect 109:325-332
Calvo, A. M., R. A. Wilson, J. W. Bok, N. P. (2012). Relationship between secondary metabolism and fungal development. Microbiology Molecular Biology Rev.66:447-459
Clay, K. 1990. Fungal endophytes of grasses. Annu, Rev. Ecol. Syst. 1990. 21:275-97
Clarah. S, R. Budihastuti, S. Darmanti (2017). Pengaruh Pupuk Nanosilika Terhadap Pertumbuhan, Ukuran Stomata dan Kandungan Klorofil Cabai Rawit Capsicum frutescens Linn) Varietas Cakra Hijau. Jurnal Biologi, Vol.6 No.2: 26-33
Costa Pinto, L., Azevedo, J.L.,Pereire, J.O., Carneiro Vieira, M.L., and Labate, C.A.A.(2000). Symtompless infection of banana and maize by endophytic fungi impairs photosynthetic efficiency. New Phytol. Vol. 147, 609-615 p
Dai C,Yu B& Li, X.2008. Screening of endophytic fungi that promote the growth of Euphorbia pekinensis, African Journal of Biotech, Vol 7, no 19,pp.3510-5
Damayanti, 2013. Potensi cendawan endofyt untuk menekan penyakit daun keriting kuning pada tanaman cabai (Capsicum annuum L.), disampaikan dalam seminar hasil penelitian Pascasarjana IPB pada tanggal 17 Januari 2013
Dearnaley, J.D.W. and A.L. Brocque. 2006. Endophytic Fungi Associated with Australian OrchidsIn Press Australian Mycologist
Fatahuddin, Amin N., D., Chandra. Y. 2003. Uji Kemampuan Beauveria bassiana Vuillemin (Hypomycetes: Moniiales) Sebagai Endofit Pada Tanaman Kubis dan Pengaruhnya terhadap Larva Plutella xylostella (Lepidoptera: Yponomeutidae). Fakultas Pertanian dan Kehutanan, Jurusan Hama dan Penyakit Tumbuhan Universitas Hasanuddin.
Gao, FK,Dai, CC & Liu, XZ 2010, Mechanism of Fungal endophytes in plant protection against pathogens, African J.Of Microbiol. Research, vol.4 no.13,pp.1351-46
Hormazabal, E, Hirschmann GS, Astudillo, L. Rodriguez J & Theoduloz, C,2005, Metabolites from Microsphaeropsis olivacea, an endophytic fungus of Pilgerodendron uviferum, Z.Naturforsch, vol.60c, pp 21-11
Jaber, l.R., and Vidal, S.(2010). Fungal endophyte negative effects on herbivory are enhanced on intact plants and maintained in a subsequent generation. Ecol. Entomol. 35, 25-36. Doi: 10.1111/j.1365-2311.2009.01152.x.
Kalteh, M., Alipour.Z.T, Ashraf, S, Ali-abadi, M.M.M Nosratabadi, A.F.2014. Effect of Silica Nanoparticles on Basil (Ocimum basilicum) Under Salinity Stress. Journal of Chemical Health Risks.4 (3): 49-55
Kartika, T. (2013). Viabilitas, parameter dan tolok ukur viabilitas benih dalam Widajati, E, Murniati, E, Palupi, ER, Kartika, T,
Suhartanto, MR & Qodir, A (eds) 1.Dasar Ilmu dan Tehnologi Benih, IPB, Press, Bogor
Kambrekar, DN. (2016). New Paradigma in exploration of microbial endophytes in insect pest management. J. Fam Sci.29 (4): 420-435
Manici, LM, Kelderexr, M, Caputo, F &Mazzola M. 2014. Auxin mediated relationships between apple plants and roof inhabiting fungi: Impact on root pathogens and potentialities of growth-promoting populations. Plant Pathol, vol.64, no.4,pp.851-43
Munandar, M., Romano & Mustafa, U. (2017). Analisis Faktor- Faktor Permintaan Cabai Merah di Kabupaten Aceh Besar. Jurnal Ilmiah Mahasiswa (JIM) Pertanian Unsyah, 2(3): 80-91
Nassar,A.H, K.A. El Tarabily and K.Sivasithamparam, (2005). Promotion of Plant Growth by An auxin-Producing Isolate of the Yeast Wiliopsis saturnus endophytic in Mayze (Zea mays L.) Roots.Biology fertile soil.Vol. 42: 97-106
Ownley B. H, Griffin M. R, Klingeman W. E, Gwinn K, Moulton J. K, Pereira R. M. 2008.Beauveria bassiana: Endophytic Colonization and Plant Disease Control. Journal Invertebrate.Pathology. 98: 267-270
Palta, P.J.(1990). Leaf chlorophyll content. Remote Sens. Rev., 207-213. Available at:http://www.tandfonline.com/doi/pdf/10.1080/027572590095321299 [Accessed Setember, 2018].
Porter, 1979.Rodriguez, R.J.White, J.F., Arnold.A.E., Redman, R.S., Jr, J.F.W, Arnold, A.E., et al. (2009).Fungal endhopytes: diversity and fungsional roles. New Phytol. 182, 314-330. Doi: 10.1111/j.1469-8137.2009.02773.x.
Raya-Diaz S, A.R Sanchez-Rodaguez, J.M. Segure-Fernandez, MdC del Campillo, and E.Quesada-Moraga. 2017. Entomopathogenic fungi-based mechanisms for improved Fe nutrition in sorghum plants grown on calcareous substrates.PloS ONE.12 910) e0185903
Saragih, M, Trizelia, Nurbailis and Yusniwati. (2019). Endophytic Colonization and Plant Growth Promoting Effect by Entomopathogenic fungus, Beauveria bassiana to Red Chili (Capsicum annuum L.) with Different Inoculation Methods. The 4th International Conference on Biological sciences and Biotechnology. IOP Conf. Series: Earth and Environmental Science 305 (2019) 012070
Singh, UB, Sahu, N, Singh, BP, Singh, HB, Manna,MC, RAo, AS & Prasad, SR. 2013,Can endophytic Arthrobotrys oligospora modulate accumulation of defence related biomolecules and induced systemic resistance intomato (Lycopersicum esculentum Mill.) against root knot disease caused by Meloydogyne incognita, Appli. Soil Ecol.,vol.63, pp.56-45.
Schulz, B., C. Boyle, 2005. The endophytic continuum. Mycological Research, 109:661-686
Sutariati, GAK & Saufan, LO. 2012. Perlakuan benih dengan rhizobakteri meningkatkan mutu benih dan hasil cabai (Capsicum annuum L.), J.Agron. Indonesia,vol 40, no 2,hlm. 131-25
Suita dan Syamsuida, 2015. Peningkatan Daya Kecepatan Berkecambah Benih. Malapari (Pongamia pinnata).
Trizelia dan Winarto. (2016). Keanekaragaman jenis cendawan entomopatogen endofit pada tanaman kakao (Theobromacacao).Pros Sem Nas MasyBiodin Indon Vol.2(2): 277-281
Trizelia, Winarto, Tanjung. A (2017). Keanekaragaman jenis cendawan endofit pada tanaman gandum (Triticum aestivum) yang berpotensi sebagai bioinsektisida. Pros Sem Nas Masy Biodiv Indon. Vol3 (3) : 433-437.
Trizelia, Martinius dan Reflinaldon (2020). The Effect of Entomopathogenic Fungi Beauveria bassiana Seed Treatment Duration on Seed Germination and Seedling Growth of Chili. Jerami Indonesian Journal of Crop Science
Tan, R.X and Zou, W.X, (2001). Endophytes: A Rich Source of Functional Metabolites. Nat Prod Rep.18: 448-459
Vega F. E, Mark S, Goettel M. S, Blackwell M, Chandler D. 2009. Fungal entomopathogens: New Insights in their Ecology. Journal Fungal Ecology. 2: 149 – 159.
Wattimena, G. A. M.Livy, Nurhayati, S. Endang, E. Andri .( 1992). Bioteknologi Tanaman. Pusat antar Universitas Bioteknologi. Bogor. Institut Pertanian Bogor
Waqas, M, Khan, AL, Kamran M, hamayun, M, Kang, SM, Kim, YH & In-Jung Lee IJ, 2012, Endophytic fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress, Molecules, vol 17,pp.10773-542012
Zabalgogeazcoa, I.(2008). Fungal endophytes and their interaction with plant pathogens. Spanish Journal of Agricultural Research 6: 138-146. DOI: http://dx.doi.org/10.5424/sjar/200806s1-382
Zheng, Y. K., Miao, c. P., Chen, H. H., huang, F. F., Xia, Y. M., Chen, Y. W., & Zhao, L. X.(2017). Endophytic fungi harbored in Panax noto ginseng: Diversity and potensial as biological control agents against host plant pathogens of root-rot disease. Journal of Ginseng Research, 41(3), 353-360. http://doi.org/10.1016/j.jgr.2016.07.005
Published
How to Cite
Issue
Section
Copyright (c) 2021 Jurnal Pertanian Tropik
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
